Metagenomics Reveals Planktonic Bacterial Community Shifts across a Natural CO2 Gradient in the Mediterranean Sea

Ashvini Chauhan; Ashish Pathak; Riccardo Rodolfo-Metalpa; Marco Milazzo; Stefan J Green; Jason M Hall-Spencer

doi:10.1128/genomeA.01543-14

Metagenomics Reveals Planktonic Bacterial Community Shifts across a Natural CO2 Gradient in the Mediterranean Sea

Genome Announc. 2015 Feb 12;3(1):e01543-14. doi: 10.1128/genomeA.01543-14.

Authors

Ashvini Chauhan¹, Ashish Pathak², Riccardo Rodolfo-Metalpa, Marco Milazzo³, Stefan J Green⁴, Jason M Hall-Spencer⁵

Affiliations

¹ School of the Environment, Florida A&M University, Tallahassee, Florida, USA ashvini.chauhan@famu.edu.
² School of the Environment, Florida A&M University, Tallahassee, Florida, USA.
³ Dipartimento di Scienze della Terra e del Mare, University of Palermo, Palermo, Italy.
⁴ DNA Services Facility, University of Illinois at Chicago, Chicago, Illinois, USA.
⁵ Marine Biology and Ecology Research Centre, Plymouth University, Plymouth, United Kingdom.

Abstract

Bacterial communities at a CO2 vent (pH 6.7) were compared with those at control (pH 8.0) and transition sites (pH 7.6) using 16S rRNA metagenomics. Firmicutes and unclassified bacteria dominated across all sites, Proteobacteria, especially Gammaproteobacteria, declined, and Epsilonproteobacteria increased in the vent with an increase in Bacteroidetes at both the vent and transition sites.