Gonad specific genes in Atlantic salmon (Salmon salar L.): characterization of tdrd7-2, dazl-2, piwil1 and tdrd1 genes

Abstract

Atlantic salmon is a commercially important species. Understanding key processes in their life history, such as germ cell development is essential for further improvements within salmon farming. Since salmonids have undergone an additional whole genome duplication compared to many other fish species, they possess more gene paralogues. Therefore, data on gene expression and function from other species may not apply for salmon. Our aim was to study the spatial and tissue specific expression of genes known from model species to be essential for germ cell development, to identify germ cell specific factors in salmon. Based on homology with other species, selected genes were predicted in the salmon genome assembly. Gene expression was measured by PCR in a variety of juvenile salmon tissues. For genes expressed exclusively in gonads we measured the expression in the same tissues as well as in eggs, embryos and larvae by qPCR. Finally, we revealed the cellular localization of the gonad specific mRNAs by in situ hybridization (ISH). Several of the selected genes (tdrd7, cxcr4b and dazl), were found in more than one copy (indicated by a number following the gene name) in the salmon genome. Expression of tdrd7-2, dazl-2, piwil1 and tdrd1 was detected exclusively in the testis and ovary of juvenile salmon, and transcripts of tdrd7-2, dazl-2 and piwil1 were localized within male and female germ cells. While tdrd7-2, piwil1 and tdrd1 were expressed in unfertilized eggs and all embryo and larval stages measured, dazl-2 was expressed in unfertilized eggs and embryos until the onset of gastrulation. This study shows that several of the genes known from model species to be essential for germ cell development, display paralogues in salmon with dissimilar and similar expression patterns in comparison to other species. Transcripts of tdrd7-2, dazl-2, piwil1 and tdrd1 are detected exclusively in gonads of juveniles and are found among maternal RNA of eggs and subsequent embryos. This information is valuable for further studies aiming at understanding salmon germ cell development.

Keywords: Gene expression; Gene paralogues; Germ cell; Ovary; Testis.