MicroRNA-145 contributes to enhancing radiosensitivity of cervical cancer cells

FEBS Lett. 2015 Mar 12;589(6):702-9. doi: 10.1016/j.febslet.2015.01.037. Epub 2015 Feb 7.

Abstract

In our study, transcriptome microarrays are used to identify differentially expressed miRNAs and mRNAs in cervical cancer specimens. We find that microRNA-145 (miR-145) expression is significantly decreased in cervical cancer tissues and cell lines, and is associated with advanced cancer stages, large tumor size and moderate/poor differentiation. We show that miR-145 targets the DNA damage repair-associated gene Helicase-like transcription factor (HLTF), which is involved in radio-resistance. Moreover, miR-145 over-expression in cervical cancer cells enhances radiosensitivity in vitro and in vivo. These results indicate that targeting miR-145 may be a novel radiosensitizing strategy for cervical cancer.

Keywords: Cervical cancer; Helicase-like transcription factor; MicroRNA-145; Microarray; Radiosensitivity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Animals
  • Base Sequence
  • Binding Sites
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism
  • Female
  • Gene Expression Regulation, Neoplastic
  • HeLa Cells
  • Humans
  • Mice, Inbred BALB C
  • Mice, Nude
  • MicroRNAs / physiology*
  • Middle Aged
  • Oligonucleotide Array Sequence Analysis
  • RNA Interference
  • Radiation Tolerance
  • Transcription Factors / genetics
  • Transcription Factors / metabolism
  • Transcriptome
  • Tumor Burden / radiation effects
  • Uterine Cervical Neoplasms / metabolism*
  • Uterine Cervical Neoplasms / pathology
  • Uterine Cervical Neoplasms / radiotherapy
  • Xenograft Model Antitumor Assays

Substances

  • DNA-Binding Proteins
  • HLTF protein, human
  • MIRN145 microRNA, human
  • MicroRNAs
  • Transcription Factors