Whole blood flow cytometric analysis of Ureaplasma-stimulated monocytes from pregnant women

Yael D Friedland; Tracey F Lee-Pullen; Elizabeth Nathan; Rory Watts; Jeffrey A Keelan; Matthew S Payne; Demelza J Ireland

doi:10.1016/j.jri.2014.12.008

Whole blood flow cytometric analysis of Ureaplasma-stimulated monocytes from pregnant women

J Reprod Immunol. 2015 Jun:109:84-8. doi: 10.1016/j.jri.2014.12.008. Epub 2015 Jan 13.

Authors

Yael D Friedland¹, Tracey F Lee-Pullen², Elizabeth Nathan³, Rory Watts⁴, Jeffrey A Keelan⁵, Matthew S Payne⁶, Demelza J Ireland⁷

Affiliations

¹ School of Women's and Infants' Health, The University of Western Australia, Perth, Western Australia, Australia. Electronic address: 10911373@student.uwa.edu.au.
² Bendat Family Comprehensive Cancer Centre, St. John of God Subiaco Hospital, Perth, Western Australia, Australia; School of Surgery, The University of Western Australia, Perth, Western Australia, Australia. Electronic address: tracey.lee-pullen@uwa.edu.au.
³ Women and Infants Research Foundation, Perth, Western Australia, Australia. Electronic address: liz.nathan@uwa.edu.au.
⁴ School of Women's and Infants' Health, The University of Western Australia, Perth, Western Australia, Australia. Electronic address: 20157536@student.uwa.edu.au.
⁵ School of Women's and Infants' Health, The University of Western Australia, Perth, Western Australia, Australia; Women and Infants Research Foundation, Perth, Western Australia, Australia. Electronic address: jeff.keelan@uwa.edu.au.
⁶ School of Women's and Infants' Health, The University of Western Australia, Perth, Western Australia, Australia. Electronic address: matthew.payne@uwa.edu.au.
⁷ School of Women's and Infants' Health, The University of Western Australia, Perth, Western Australia, Australia. Electronic address: demelza.ireland@uwa.edu.au.

PMID: 25661326
DOI: 10.1016/j.jri.2014.12.008

Abstract

We hypothesised that circulating monocytes of women with vaginal colonisation with Ureaplasma spp., genital microorganisms known to cause inflammation-driven preterm birth, would elicit a tolerised cytokine response to subsequent in vitro Ureaplasma parvum serovar 3 (UpSV3) stimulation. Using multi-parameter flow cytometry, we found no differences with regard to maternal colonisation status in the frequency of TNF-α-, IL-6-, IL-8- and IL-1β-expressing monocytes in response to subsequent UpSV3 stimulation (P > 0.10 for all cytokines). We conclude that vaginal Ureaplasma spp. colonisation does not specifically tolerise monocytes of pregnant women towards decreased responses to subsequent stimulation.

Keywords: Cytokines; Monocytes; Multi-parameter flow cytometry; Preterm birth; Ureaplasma spp..

Publication types

Clinical Trial
Research Support, Non-U.S. Gov't

MeSH terms

Adult
Female
Flow Cytometry*
Humans
Monocytes / immunology*
Monocytes / metabolism
Monokines / blood
Monokines / immunology*
Pregnancy
Premature Birth / blood
Premature Birth / immunology
Premature Birth / microbiology
Ureaplasma / immunology*

Substances

Monokines