Controlled uptake and release of lysozyme from glycerol diglycidyl ether cross-linked oxidized starch microgel

Luhai Zhao; Yuying Chen; Wei Li; Meiling Lu; Shanshan Wang; Xiaodong Chen; Mengxuan Shi; Jiande Wu; Qipeng Yuan; Yuan Li

doi:10.1016/j.carbpol.2015.01.002

Controlled uptake and release of lysozyme from glycerol diglycidyl ether cross-linked oxidized starch microgel

Carbohydr Polym. 2015 May 5:121:276-83. doi: 10.1016/j.carbpol.2015.01.002. Epub 2015 Jan 7.

Authors

Luhai Zhao¹, Yuying Chen¹, Wei Li¹, Meiling Lu¹, Shanshan Wang¹, Xiaodong Chen¹, Mengxuan Shi¹, Jiande Wu¹, Qipeng Yuan¹, Yuan Li²

Affiliations

¹ State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, College of Life Science and Technology, P. O. Box 53, 100029 Beijing, China.
² State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, College of Life Science and Technology, P. O. Box 53, 100029 Beijing, China. Electronic address: liyuan@mail.buct.edu.cn.

PMID: 25659699
DOI: 10.1016/j.carbpol.2015.01.002

Abstract

A biodegradable microgel system based on glycerol-1,3-diglycidyl ether (GDGE) cross-linked TEMPO-oxidized potato starch polymers was developed for controlled uptake and release of proteins. A series of microgels were prepared with a wide range of charge density and cross-link density. We found both swelling capacity (SWw) and lysozyme uptake at saturation (Γsat) increased with increasing degree of oxidation (DO) and decreasing cross-link density. Microgel of DO100% with a low cross-link density (RGDGE/polymer (w/w) of 0.025) was selected to be the optimum gel type for lysozyme absorption; Γsat increased with increasing pH and decreasing ionic strength. It suggests that the binding strength was the strongest at high pH and low ionic strength, which was recognized as the optimum absorption conditions. The lysozyme release was promoted at low pH and high ionic strength, which were considered to be the most suitable conditions for triggering protein release. These results may provide useful information for the controlled uptake and release of proteins by oxidized starch microgels.

Keywords: Controlled release; Lysozyme; Oxidized starch microgels; Protein absorption; TEMPO-oxidation; pH responsive.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cross-Linking Reagents / chemistry
Epoxy Compounds / chemistry*
Gels / chemistry*
Glyceryl Ethers / chemistry*
Muramidase / chemistry*
Oxidation-Reduction
Starch / chemistry*

Substances

Cross-Linking Reagents
Epoxy Compounds
Gels
Glyceryl Ethers
glycerol 1,3-diglycidyl ether
Starch
Muramidase