Lysozyme depolymerization of photo-activated chitosan adhesive films

Damia Mawad; Charles Warren; Mathew Barton; David Mahns; John Morley; Binh T T Pham; Nguyen T H Pham; Sindy Kueh; Antonio Lauto

doi:10.1016/j.carbpol.2014.12.008

Lysozyme depolymerization of photo-activated chitosan adhesive films

Carbohydr Polym. 2015 May 5:121:56-63. doi: 10.1016/j.carbpol.2014.12.008. Epub 2014 Dec 31.

Authors

Damia Mawad¹, Charles Warren², Mathew Barton³, David Mahns³, John Morley³, Binh T T Pham⁴, Nguyen T H Pham⁴, Sindy Kueh³, Antonio Lauto⁵

Affiliations

¹ Department of Materials, Department of Bioengineering and Institute for Biomedical Engineering, Imperial College London, Exhibition Road, London SW7 2AZ, UK.
² School of Biological Sciences, The University of Sydney, Sydney 2006, NSW, Australia.
³ School of Medicine, University of Western Sydney, Locked Bag 1797, Penrith 2751, NSW, Australia.
⁴ Key Centre for Polymers and Colloids, School of Chemistry, University of Sydney, Sydney 2006, NSW, Australia.
⁵ School of Medicine, University of Western Sydney, Locked Bag 1797, Penrith 2751, NSW, Australia; Biomedical Engineering & Neuroscience (BENS) Research Group, The MARCS Institute, University of Western Sydney, Locked Bag 1797, Penrith 2751, NSW, Australia. Electronic address: a.lauto@uws.edu.au.

PMID: 25659671
DOI: 10.1016/j.carbpol.2014.12.008

Abstract

Effective tissue bioadhesion of rose bengal-chitosan films can be achieved by photoactivation using a green laser. In this study, lysozyme was incorporated in these films to enhance the rate of depolymerization and assess the laser impact on lysozyme. The lysozyme loaded films exhibited a 21% mass loss after 4 weeks implantation in rats while control films (without lysozyme) had only 7% mass loss. Capillary electrophoresis-mass spectroscopy showed that chitosan degraded into monomers and oligomers of glucosamine and N-acetyl-glucosamine. Irradiation with laser did not affect the depolymerization of adhesive by lysozyme suggesting that the inclusion of lysozyme in the bioadhesive is a viable technique for tailoring the depolymerization.

Keywords: Capillary electrophoresis; Laser; Lysozyme; Mass spectroscopy.

MeSH terms

Adhesives / chemistry*
Adhesives / metabolism
Animals
Biocompatible Materials / chemistry
Biocompatible Materials / metabolism
Chitosan / chemistry*
Chitosan / metabolism
Female
Lasers*
Muramidase / metabolism*
Polymerization*
Rats
Rose Bengal / chemistry

Substances

Adhesives
Biocompatible Materials
Rose Bengal
Chitosan
Muramidase