18F-FPRGD2 PET/CT imaging of integrin αvβ3 in renal carcinomas: correlation with histopathology

Nadia Withofs; Nicolas Signolle; Joan Somja; Pierre Lovinfosse; Eugène Mutijima Nzaramba; Frédéric Mievis; Fabrice Giacomelli; David Waltregny; Didier Cataldo; Sanjiv S Gambhir; Roland Hustinx

doi:10.2967/jnumed.114.149021

18F-FPRGD2 PET/CT imaging of integrin αvβ3 in renal carcinomas: correlation with histopathology

J Nucl Med. 2015 Mar;56(3):361-4. doi: 10.2967/jnumed.114.149021. Epub 2015 Feb 5.

Affiliations

¹ Nuclear Medicine and Oncological Imaging Division, Medical Physics Department, CHU Liege, Liege, Belgium nwithofs@chu.ulg.ac.be.
² Laboratory of Tumor and Developmental Biology, GIGA Research, University of Liege, Liege, Belgium.
³ Pathology Department, CHU Liege, Liege, Belgium.
⁴ Nuclear Medicine and Oncological Imaging Division, Medical Physics Department, CHU Liege, Liege, Belgium.
⁵ Cyclotron Research Center, University of Liege, Liege, Belgium.
⁶ Urology Department, CHU Liege, Liege, Belgium; and.
⁷ Molecular Imaging Program at Stanford (MIPS), Radiology Department, Stanford University, Stanford, California.

PMID: 25655629
DOI: 10.2967/jnumed.114.149021

Abstract

This study aimed to correlate (18)F-FB-mini-PEG-E[c(RGDyK)](2) ((18)F-FPRGD2) uptake to integrin αvβ3 expression and angiogenesis in renal tumors.

Methods: (18)F-FPRGD2 PET/CT was performed on 27 patients before surgical resection (median 4 d) of a renal mass. The (18)F-FPRGD2 uptake was compared with integrin αvβ3, CD31, CD105, and Ki-67 using immunohistochemistry; with placental growth factor and vascular endothelial growth factor receptors 1 and 2 using reverse transcription polymerase chain reaction; and with vascular endothelial growth factor A isoforms using enzyme-linked immunosorbent assay.

Results: Overall, (18)F-FPRGD2 uptake significantly correlated (P < 0.0001) with integrin αvβ3 expression in renal masses. However, it correlated only with integrin αvβ3-positive vessels in the group of papillary carcinomas whereas it correlated with integrin αvβ3 expression by tumor cells in the clear cell carcinoma group.

Conclusion: (18)F-FPRGD2 uptake reflects the expression of integrin αvβ3 in renal tumors but represents angiogenesis only when tumor cells do not express the integrin.

Keywords: PET; RGD; angiogenesis; integrin; renal cancer.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aged
Carcinoma, Renal Cell / diagnostic imaging*
Cell Membrane / metabolism
Female
Humans
Integrin alphaVbeta3 / metabolism*
Kidney Neoplasms / diagnostic imaging*
Male
Microcirculation
Middle Aged
Neovascularization, Pathologic
Nuclear Medicine
Peptides, Cyclic*
Platelet Endothelial Cell Adhesion Molecule-1 / metabolism
Polyethylene Glycols / chemistry
Prospective Studies
Radionuclide Imaging
Radiopharmaceuticals
Tomography, X-Ray Computed
Treatment Outcome

Substances

4-fluorobenozyl-glutamyl-cyclo(arginyl-glycyl-aspartyl-tyrosyl-lysyl)2
Integrin alphaVbeta3
Peptides, Cyclic
Platelet Endothelial Cell Adhesion Molecule-1
Radiopharmaceuticals
Polyethylene Glycols