Direct observation of the growth and shrinkage of microtubules by single molecule Förster resonance energy transfer

Prasenjit Mondal; Shyamtanu Chattoraj; Rajdeep Chowdhury; Debmalya Bhunia; Surajit Ghosh; Kankan Bhattacharyya

doi:10.1039/c4cp06031h

Direct observation of the growth and shrinkage of microtubules by single molecule Förster resonance energy transfer

Phys Chem Chem Phys. 2015 Mar 14;17(10):6687-90. doi: 10.1039/c4cp06031h.

Authors

Prasenjit Mondal¹, Shyamtanu Chattoraj, Rajdeep Chowdhury, Debmalya Bhunia, Surajit Ghosh, Kankan Bhattacharyya

Affiliation

¹ Chemistry Division, CSIR-Indian Institute of Chemical Biology, Jadavpur, Kolkata-700032, India. sghosh@iicb.res.in.

PMID: 25655478
DOI: 10.1039/c4cp06031h

Abstract

Single molecule Förster resonance energy transfer (FRET) has been applied, for the first time, to monitor the growth (polymerization) and the shrinkage (depolymerization) of the dynamic microtubules, employing EGFP (attached to Mal3) as a donor and alexa-568 bound to tubulin as an acceptor.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Fluorescence Resonance Energy Transfer*
Green Fluorescent Proteins / chemistry
Green Fluorescent Proteins / metabolism
Microtubules / chemistry
Microtubules / metabolism*
Tubulin / chemistry
Tubulin / metabolism

Substances

Tubulin
enhanced green fluorescent protein
Green Fluorescent Proteins