Insecticidal and repellent activity of Siparuna guianensis Aubl. (Negramina) against Aedes aegypti and Culex quinquefasciatus

Raimundo Wagner Souza Aguiar; Suetonio Fernandes dos Santos; Fabricio da Silva Morgado; Sergio Donizeti Ascencio; Magnólia de Mendonça Lopes; Kelvinson Fernandes Viana; Julcemar Didonet; Bergmann Morais Ribeiro

doi:10.1371/journal.pone.0116765

Insecticidal and repellent activity of Siparuna guianensis Aubl. (Negramina) against Aedes aegypti and Culex quinquefasciatus

PLoS One. 2015 Feb 3;10(2):e0116765. doi: 10.1371/journal.pone.0116765. eCollection 2015.

Authors

Raimundo Wagner Souza Aguiar¹, Suetonio Fernandes dos Santos¹, Fabricio da Silva Morgado², Sergio Donizeti Ascencio³, Magnólia de Mendonça Lopes¹, Kelvinson Fernandes Viana¹, Julcemar Didonet¹, Bergmann Morais Ribeiro²

Affiliations

¹ Universidade Federal do Tocantins, Departamento de Biotecnologia, Campus Universitário de Gurupi, Gurupi, Tocantins, Brazil.
² Universidade de Brasília, Departamento de Biologia Celular, Campus Universitário, Asa Norte, Brasília, Distrito Federal, Brazil.
³ Universidade Federal do Tocantins, Campus Universitário de Palmas, Tocantins, Brazil.

Abstract

This study investigated the toxic effects of essential oils isolated from Siparuna guianensis against Aedes aegypti, Culex quinquefasciatus (eggs, larvae, pupae, and adult) and Aedes albopictus (C6/36) cells. The oviposition-deterring activity, egg viability, and repellence activity in the presence of different essential oils concentrations were determined. The essential oils showed high toxicity to all developmental stages of A. aegypti and C. quinquefasciatus. Furthermore, the oils also showed high repellent activity towards the adult stage of mosquitoes (0.025 to 0.550 μg/cm2 skin conferred 100% repellence up to 120 min) and in contact with cultured insect cells (C6/36) induced death possibly by necrosis. The results presented in this work show the potential of S. guianensis essential oils for the development of an alternative and effective method for the natural control of mosquitoes in homes and urban areas.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aedes / drug effects*
Aedes / physiology
Animals
Cell Line
Culex / drug effects*
Culex / physiology
Fruit / chemistry
Insect Repellents / isolation & purification
Insect Repellents / toxicity*
Insecticides* / isolation & purification
Larva / drug effects
Magnoliopsida / chemistry*
Mosquito Control
Oils, Volatile / isolation & purification
Oils, Volatile / toxicity*
Oviposition / drug effects
Ovum / drug effects
Ovum / physiology
Plant Leaves / chemistry
Plant Stems / chemistry

Substances

Insect Repellents
Insecticides
Oils, Volatile

Grants and funding

This work was supported by CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico), www.cnpq.br, SECT (Secretária de Ciência Tecnologia do estado do Tocantins), http://www.sedecti.to.gov.br/sedecti/, and CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior), www.capes.gov.br. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.