Evaluation of human and microbial DNA content in subgingival plaque samples collected by paper points or curette

P J Pérez-Chaparro; P M Duarte; C M Pannuti; L C Figueiredo; M J Mestnik; C P S Gonçalves; M Faveri; M Feres

doi:10.1016/j.mimet.2015.01.023

Evaluation of human and microbial DNA content in subgingival plaque samples collected by paper points or curette

J Microbiol Methods. 2015 Apr:111:19-20. doi: 10.1016/j.mimet.2015.01.023. Epub 2015 Jan 30.

Authors

P J Pérez-Chaparro¹, P M Duarte², C M Pannuti³, L C Figueiredo², M J Mestnik², C P S Gonçalves², M Faveri², M Feres²

Affiliations

¹ Department of Periodontology, Dental Research Division, Guarulhos University, Sao Paulo, Brazil. Electronic address: julianaperezch@gmail.com.
² Department of Periodontology, Dental Research Division, Guarulhos University, Sao Paulo, Brazil.
³ Department of Stomatology, School of Dentistry, University of São Paulo, São Paulo, Brazil.

PMID: 25644890
DOI: 10.1016/j.mimet.2015.01.023

Abstract

Host DNA may adversely affect metagenomic studies focusing on the prokaryotic microbiota. This study compared the levels of host DNA in subgingival plaque collected by paper points and curette, using quantitative PCR. Lower proportions of host DNA and higher proportions of bacterial DNA were recovered from samples collected with curettes.

Keywords: Dental plaque; High-Throughput Nucleotide Sequencing; Microbiota; Quantitative Real-Time Polymerase Chain Reaction.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Chronic Periodontitis / microbiology
DNA / isolation & purification*
DNA, Bacterial / isolation & purification*
Dental Plaque / microbiology*
High-Throughput Nucleotide Sequencing
Humans
Metagenomics
Microbiota
Paper
Real-Time Polymerase Chain Reaction*
Specimen Handling / methods

Substances

DNA, Bacterial
DNA