Proteomics-driven analysis of ovine whey colostrum

PLoS One. 2015 Feb 2;10(2):e0117433. doi: 10.1371/journal.pone.0117433. eCollection 2015.

Abstract

The aim of this study was to shed light in to the complexity of the ovine colostrum proteome, with a specific focus on the low abundance proteins. The ovine colostrum is characterized by a few dominating proteins, as the immunoglobulins, but it also contains less represented protein species, equally important for the correct development of neonates. Ovine colostrum, collected immediately after lambing, was separated by 1D SDS-PAGE. Proteins bands were digested with trypsin and the resulting peptides were analyzed by LC-MS/MS. On the basis of the Swiss-Prot database, a total of 343 unique proteins were identified. To our knowledge, this study represents the most comprehensive analysis of ovine colostrum proteome.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Colostrum / metabolism*
  • Female
  • Gene Expression Regulation
  • Gene Ontology
  • Lactation
  • Milk Proteins / genetics
  • Milk Proteins / metabolism
  • Pregnancy
  • Proteomics*
  • Sheep / genetics
  • Sheep / growth & development
  • Sheep / metabolism*
  • Sheep / physiology
  • Whey Proteins

Substances

  • Milk Proteins
  • Whey Proteins

Grants and funding

This work was supported by progetto special “ricerca di eccellenza anno 2011” Fondazione cassa di risparmio della provincial di Teramo. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.