Preparation of uniform-sized hemoglobin-albumin microspheres as oxygen carriers by Shirasu porous glass membrane emulsification technique

Colloids Surf B Biointerfaces. 2015 Mar 1:127:1-7. doi: 10.1016/j.colsurfb.2015.01.018. Epub 2015 Jan 19.

Abstract

We have developed a new type of artificial oxygen carrier composed of bovine hemoglobin (bHb) and bovine serum albumin (BSA) prepared by Shirasu porous glass (SPG) membrane emulsification technique. The resultant emulsion droplets containing 10 wt% bHb and 5-20 wt% BSA were subsequently cross-linked by glutaraldehyde to form the microspheres. Due to the uniform pore structure of SPG membranes, the average diameters of bHb10-BSAm microspheres were successfully controlled at around 5 μm with a coefficient of variation of around 10%. In addition, the biocompatibility of the carriers depended on their oxyhemoglobin percentage regardless of their same size. Finally, the P50 values of these microspheres ranged from 8.08 to 11.60 mmHg, which showed a high oxygen affinity and an oxygen delivery function.

Keywords: Albumin; Hemoglobin; Membrane emulsification; Oxygen carriers; Shirasu porous glass membrane.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cattle
  • Cell Survival
  • Drug Compounding / methods*
  • Emulsions / chemistry*
  • Glass / chemistry*
  • Hemoglobins / chemistry*
  • Hep G2 Cells
  • Human Umbilical Vein Endothelial Cells
  • Humans
  • Membranes, Artificial
  • Microscopy, Electron, Scanning
  • Microspheres*
  • Optical Imaging
  • Oxygen / chemistry*
  • Particle Size*
  • Porosity
  • Protein Stability
  • Serum Albumin, Bovine / chemistry*
  • Shear Strength
  • Spectrophotometry, Ultraviolet
  • Viscosity

Substances

  • Emulsions
  • Hemoglobins
  • Membranes, Artificial
  • Serum Albumin, Bovine
  • Oxygen