Multiplex nucleic acid suspension bead arrays for detection and subtyping of filoviruses

Cecilia Bergqvist; Petra Holmström; Gunnel Lindegren; Nina Lagerqvist; Mikael Leijon; Kerstin I Falk

doi:10.1128/JCM.02787-14

Multiplex nucleic acid suspension bead arrays for detection and subtyping of filoviruses

J Clin Microbiol. 2015 Apr;53(4):1368-70. doi: 10.1128/JCM.02787-14. Epub 2015 Jan 28.

Authors

Cecilia Bergqvist¹, Petra Holmström², Gunnel Lindegren², Nina Lagerqvist², Mikael Leijon³, Kerstin I Falk⁴

Affiliations

¹ Public Health Agency of Sweden, Solna, Sweden Stockholm University, Stockholm, Sweden.
² Public Health Agency of Sweden, Solna, Sweden.
³ National Veterinary Institute, Uppsala, Sweden.
⁴ Public Health Agency of Sweden, Solna, Sweden Karolinska Institutet, Stockholm, Sweden kerstin.falk@folkhalsomyndigheten.se.

Abstract

Here we describe multiplex suspension bead array systems that allow fast and reliable detection of reverse transcriptase (RT) PCR amplified filovirus genomes and also enable subtyping of Ebola virus species and Marburg virus strains. These systems have an analytical sensitivity equivalent to that of RT-PCR.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Ebolavirus / genetics*
Ebolavirus / isolation & purification*
Humans
Marburgvirus / genetics*
Marburgvirus / isolation & purification*
Nucleic Acid Amplification Techniques / instrumentation*
Nucleic Acid Amplification Techniques / methods*
Reverse Transcriptase Polymerase Chain Reaction
Sensitivity and Specificity