Live-cell assessment of mitochondrial reactive oxygen species using dihydroethidine

Marleen Forkink; Peter H G M Willems; Werner J H Koopman; Sander Grefte

doi:10.1007/978-1-4939-2257-4_15

Live-cell assessment of mitochondrial reactive oxygen species using dihydroethidine

Methods Mol Biol. 2015:1264:161-9. doi: 10.1007/978-1-4939-2257-4_15.

Authors

Marleen Forkink¹, Peter H G M Willems, Werner J H Koopman, Sander Grefte

Affiliation

¹ Department of Biochemistry, Raboud Institute for Molecular Life Sciences, Radboud University Medical Centre, P.O. Box 9101, NL-6500 HB, Nijmegen, The Netherlands.

PMID: 25631012
DOI: 10.1007/978-1-4939-2257-4_15

Abstract

Reactive oxygen species (ROS) play an important role in both physiology and pathology. Mitochondria are an important source of the primary ROS superoxide. However, accurate detection of mitochondrial superoxide especially in living cells remains a difficult task. Here, we describe a method and the pitfalls to detect superoxide in both mitochondria and the entire cell using dihydroethidium (HEt) and live-cell microscopy.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Line
Dicarbethoxydihydrocollidine / analogs & derivatives*
Dicarbethoxydihydrocollidine / metabolism
Humans
Image Processing, Computer-Assisted / methods
Microscopy / methods*
Mitochondria / metabolism*
Molecular Imaging / methods
Oxidation-Reduction
Reactive Oxygen Species / metabolism*

Substances

Reactive Oxygen Species
dihydroethidine
Dicarbethoxydihydrocollidine