Evaluation of the effects of quercetin on damaged salivary secretion

Ayako Takahashi; Hiroko Inoue; Kenji Mishima; Fumio Ide; Ryoko Nakayama; Ayaka Hasaka; Koufuchi Ryo; Yumi Ito; Takashi Sakurai; Yoshinori Hasegawa; Ichiro Saito

doi:10.1371/journal.pone.0116008

Evaluation of the effects of quercetin on damaged salivary secretion

PLoS One. 2015 Jan 28;10(1):e0116008. doi: 10.1371/journal.pone.0116008. eCollection 2015.

Authors

Affiliations

¹ Department of Pathology, Tsurumi University School of Dental Medicine, Yokohama, Japan.
² Department of Pathology, Tsurumi University School of Dental Medicine, Yokohama, Japan; Department of Pharmaceutical Sciences, Nihon Pharmaceutical University, Saitama, Japan.
³ Division of Pathology, Department of Oral Diagnostic Sciences, School of Dentistry, Showa University, Tokyo, Japan.
⁴ Department of Radiopraxis Science, Graduate School of Dentistry, Kanagawa Dental University, Yokosuka, Japan.
⁵ Department of Human Genome Research, Kazusa DNA Research Institute, Chiba, Japan.

Abstract

With the aim of discovering an effective method to treat dry mouth, we analyzed the effects of quercetin on salivary secretion and its mechanism of action. We created a mouse model with impaired salivary secretion by exposure to radiation and found that impaired secretion is suppressed by quercetin intake. Moreover, secretion levels were enhanced in quercetin-fed normal mice. To elucidate the mechanisms of these effects on salivary secretion, we conducted an analysis using mouse submandibular gland tissues, a human salivary gland epithelial cell line (HSY), and mouse aortic endothelial cells (MAECs). The results showed that quercetin augments aquaporin 5 (AQP5) expression and calcium uptake, and suppresses oxidative stress and inflammatory responses induced by radiation exposure, suggesting that quercetin intake may be an effective method to treat impaired salivary secretion.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antioxidants / pharmacology*
Calcium / metabolism
Cytokines / genetics
Cytokines / metabolism
Disease Models, Animal
Gene Expression Profiling
Gene Expression Regulation / drug effects
Gene Regulatory Networks
Inflammation Mediators / metabolism
Intracellular Space / metabolism
Male
Mice
Nitric Oxide Synthase Type III / genetics
Nitric Oxide Synthase Type III / metabolism
Quercetin / pharmacology*
Radiation Injuries, Experimental
Saliva
Salivary Glands / drug effects*
Salivary Glands / metabolism*
Salivary Glands / pathology
Salivary Glands / radiation effects
Salivation / drug effects*
Salivation / genetics

Substances

Antioxidants
Cytokines
Inflammation Mediators
Quercetin
Nitric Oxide Synthase Type III
Calcium

Grants and funding

This work was supported by a grant for a research project towards the creation of new agricultural products from the Ministry of Agriculture, Forestry and Fisheries of Japan.