Remodeling of phospholipid composition in colon cancer cells by 1α,25(OH)2D3 and its analogs

Carlien Leyssens; Eyra Marien; Lieve Verlinden; Rita Derua; Etienne Waelkens; Johannes V Swinnen; Annemieke Verstuyf

doi:10.1016/j.jsbmb.2015.01.018

Remodeling of phospholipid composition in colon cancer cells by 1α,25(OH)2D3 and its analogs

J Steroid Biochem Mol Biol. 2015 Apr:148:172-8. doi: 10.1016/j.jsbmb.2015.01.018. Epub 2015 Jan 24.

Authors

Carlien Leyssens¹, Eyra Marien², Lieve Verlinden³, Rita Derua⁴, Etienne Waelkens⁵, Johannes V Swinnen⁶, Annemieke Verstuyf⁷

Affiliations

¹ Laboratory of Clinical and Experimental Endocrinology, KU Leuven - University of Leuven, Herestraat 49, bus 902, 3000 Leuven, Belgium. Electronic address: carlien.leyssens@med.kuleuven.be.
² Laboratory of Lipid Metabolism and Cancer, KU Leuven - University of Leuven, Herestraat 49, bus 902, 3000 Leuven, Belgium. Electronic address: eyra.marien@med.kuleuven.be.
³ Laboratory of Clinical and Experimental Endocrinology, KU Leuven - University of Leuven, Herestraat 49, bus 902, 3000 Leuven, Belgium. Electronic address: lieve.verlinden@med.kuleuven.be.
⁴ Laboratory of Protein Phosphorylation and Proteomics, KU Leuven - University of Leuven, Herestraat 49, bus 901, 3000 Leuven, Belgium. Electronic address: rita.derua@med.kuleuven.be.
⁵ Laboratory of Protein Phosphorylation and Proteomics, KU Leuven - University of Leuven, Herestraat 49, bus 901, 3000 Leuven, Belgium. Electronic address: etienne.waelkens@med.kuleuven.be.
⁶ Laboratory of Lipid Metabolism and Cancer, KU Leuven - University of Leuven, Herestraat 49, bus 902, 3000 Leuven, Belgium. Electronic address: johan.swinnen@med.kuleuven.be.
⁷ Laboratory of Clinical and Experimental Endocrinology, KU Leuven - University of Leuven, Herestraat 49, bus 902, 3000 Leuven, Belgium. Electronic address: mieke.verstuyf@med.kuleuven.be.

PMID: 25625664
DOI: 10.1016/j.jsbmb.2015.01.018

Abstract

Alterations in cellular phospholipid composition are emerging as important traits in the development and progression of cancer. In this study we investigated the effects of 1α,25-dihydroxyvitamin D3 [1,25(OH)2D3] and two of its more antiproliferative analogs on the cellular phospholipid composition of various human colon cancer cell lines. Treatment of Caco-2, SW1417 and SW480-ADH cells with 3×10(-8)M 1,25(OH)2D3, CD578 or WU515 evoked significant changes in phospholipid composition, with the analogs being more potent than the natural compound. Observed effects included changes in acyl chain elongation and acyl chain saturation, and were substantially different in the various cell lines. Consistent with the alterations in phospholipid profiles, 1,25(OH)2D3 and its analogs provoked changes in several lipogenic enzymes such as fatty acid synthase (FASN), acetyl-CoA carboxylase (ACACA) and fatty acid elongases (ELOVLs). These effects were also cell line dependent. Taken together these findings indicate that 1,25(OH)2D3 and its analogs have divergent effects on the phospholipid composition of different colon cancer cell lines and warrant further investigation of the effect of 1,25(OH)2D3 and its analogs on lipid metabolism in various subtypes of primary human colon cancers.

Keywords: Colon cancer; Lipidomics; Phospholipid metabolism; Vitamin D; Vitamin D analogs.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Calcitriol / analogs & derivatives*
Calcitriol / pharmacology*
Colonic Neoplasms / drug therapy*
Colonic Neoplasms / metabolism*
Humans
Phospholipids / metabolism*
Tumor Cells, Cultured
Vitamins / pharmacology*

Substances

Phospholipids
Vitamins
Calcitriol