Distributional variations in trabecular architecture of the mandibular bone: an in vivo micro-CT analysis in rats

Zhongshuang Liu; Chengwei Yan; Chen Kang; Bin Zhang; Ying Li

doi:10.1371/journal.pone.0116194

Distributional variations in trabecular architecture of the mandibular bone: an in vivo micro-CT analysis in rats

PLoS One. 2015 Jan 27;10(1):e0116194. doi: 10.1371/journal.pone.0116194. eCollection 2015.

Authors

Zhongshuang Liu¹, Chengwei Yan², Chen Kang¹, Bin Zhang³, Ying Li³

Affiliations

¹ Sino-Russian Institute of Hard Tissue Development and Regeneration, the Second Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang, China.
² Department of Pediatric Dentistry, the First Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang, China.
³ Sino-Russian Institute of Hard Tissue Development and Regeneration, the Second Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang, China; Heilongjiang Academy of Medical Sciences, Harbin 150001, Heilongjiang, China.

Abstract

Purpose: To evaluate the effect of trabecular thickness and trabecular separation on modulating the trabecular architecture of the mandibular bone in ovariectomized rats.

Materials and methods: Fourteen 12-week-old adult female Wistar rats were divided into an ovariectomy group (OVX) and a sham-ovariectomy group (sham). Five months after the surgery, the mandibles from 14 rats (seven OVX and seven sham) were analyzed by micro-CT. Images of inter-radicular alveolar bone of the mandibular first molars underwent three-dimensional reconstruction and were analyzed.

Results: Compared to the sham group, trabecular thickness in OVX alveolar bone decreased by 27% (P = 0.012), but trabecular separation in OVX alveolar bone increased by 59% (P = 0.005). A thickness and separation map showed that trabeculae of less than 100 μm increased by 46%, whereas trabeculae of more than 200 μm decreased by more than 40% in the OVX group compared to those in the sham group. Furthermore, the OVX separation of those trabecular of more than 200 μm was 65% higher compared to the sham group. Bone mineral density (P = 0.028) and bone volume fraction (p = 0.001) were also significantly decreased in the OVX group compared to the sham group.

Conclusions: Ovariectomy-induced bone loss in mandibular bone may be related to the distributional variations in trabecular thickness and separation which profoundly impact the modulation of the trabecular architecture.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bone Density
Female
Humans
Mandible / diagnostic imaging*
Mandible / pathology
Osteoporosis, Postmenopausal / diagnostic imaging*
Osteoporosis, Postmenopausal / pathology
Rats, Wistar
X-Ray Microtomography

Grants and funding

This work was supported by the Heilongjiang postgraduate innovation science foundation (Grant No. YJSCX2012-240HLJ) and Natural Science Foundation of China (Grant No. 81170960), the National Key Science Instrument and Equipment Development Project (Grant No. 2011YQ04008708), and Specialized Research Fund for the Doctoral Program of Higher Education (Grant No. 20102307110006). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.