Comparative analysis of monoclonal antibody N-glycosylation using stable isotope labelling and UPLC-fluorescence-MS

Silvia Millán Martín; Cédric Delporte; Amy Farrell; Natalia Navas Iglesias; Niaobh McLoughlin; Jonathan Bones

doi:10.1039/c4an02345e

Comparative analysis of monoclonal antibody N-glycosylation using stable isotope labelling and UPLC-fluorescence-MS

Analyst. 2015 Mar 7;140(5):1442-7. doi: 10.1039/c4an02345e. Epub 2015 Jan 27.

Authors

Silvia Millán Martín¹, Cédric Delporte, Amy Farrell, Natalia Navas Iglesias, Niaobh McLoughlin, Jonathan Bones

Affiliation

¹ Characterisation and Comparability Laboratory, NIBRT - The National Institute for Bioprocessing Research and Training, Foster Avenue, Mount Merrion, Blackrock, Co., Dublin, Ireland. jonathan.bones@nibrt.ie.

PMID: 25623139
DOI: 10.1039/c4an02345e

Abstract

A twoplex method using (12)C6 and (13)C6 stable isotope analogues (Δmass = 6 Da) of 2-aminobenzoic acid (2-AA) is described for quantitative analysis of N-glycans present on monoclonal antibodies and other glycoproteins using ultra performance liquid chromatography with sequential fluorescence and accurate mass tandem quadrupole time of flight (QToF) mass spectrometric detection.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Antibodies, Monoclonal / chemistry*
Carbon Radioisotopes
Chromatography, Liquid / methods*
Glycoproteins / chemistry*
Glycosylation
Humans
Isotope Labeling / methods*
Polysaccharides / analysis*
Tandem Mass Spectrometry / methods*
ortho-Aminobenzoates / chemistry*

Substances

Antibodies, Monoclonal
Carbon Radioisotopes
Glycoproteins
Polysaccharides
ortho-Aminobenzoates
anthranilic acid