Synthesis and characterization of water-soluble, heteronuclear ruthenium(III)/ferrocene complexes and their interactions with biomolecules

J Inorg Biochem. 2015 Apr:145:41-50. doi: 10.1016/j.jinorgbio.2014.12.017. Epub 2014 Dec 24.

Abstract

The reaction of Na[RuCl4(SO(CH3)2)2], 1, with one equivalent of FcCONHCH2C6H4N (Fc=FeC10H9), L1, FcCOOCH2CH2C3H3N2, L2, FcCOOC6H4N, L3, afforded the dinuclear species, Na[FcCONHCH2C6H4N[RuCl4(SO(CH3)2)]], RuL1, Na[FcCOOCH2CH2C3H3N2[RuCl4(SO(CH3)2)]], RuL2, Na[FcCOOC6H4N(RuCl4(SO(CH3)2))], RuL3, respectively, yielding, in each case, a ferrocene moiety bridged to a ruthenium center. The complexes were characterized by NMR, IR, and XRD (X-ray diffraction). The sulfoxide ligands are bonded to the metal through the sulfur atom. The complexes were evaluated for their biological activity with pBluescript DNA plasmid, and the protein BSA (bovine serum albumin). These reactions were monitored by XAS (X-ray absorption spectroscopy), EXAFS (extended X-ray Absorption Fine Structure), NMR, UV/visible, emission spectroscopy, and gel electrophoresis. Donor atoms from the biomolecules substitute for the chloride ligands in the parent complexes.

Keywords: Cancer; Ferrocene; NMR; Paramagnetic; Ruthenium.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • DNA / chemistry*
  • Ferrous Compounds / chemical synthesis*
  • Ferrous Compounds / chemistry
  • Metallocenes
  • Models, Molecular
  • Ruthenium Compounds / chemical synthesis*
  • Ruthenium Compounds / chemistry
  • Serum Albumin, Bovine / chemistry*
  • Solubility
  • Spectrum Analysis / methods
  • Water / chemistry
  • X-Ray Diffraction

Substances

  • Ferrous Compounds
  • Metallocenes
  • Ruthenium Compounds
  • Water
  • Serum Albumin, Bovine
  • DNA
  • ferrocene