Subtyping of Clostridium difficile is crucial for outbreak investigations. An extended multilocus variable-number tandem-repeat analysis (eMLVA) of 14 variable number tandem repeat (VNTR) loci was validated in multiplex format compatible with a routine typing laboratory and showed excellent concordance with tandem repeat sequence typing (TRST) and high discriminatory power.
Keywords: Clostridium difficile; Molecular subtyping; Multilocus variable-number tandem-repeat analysis; Tandem repeat sequence typing.
Copyright © 2015. Published by Elsevier B.V.