Novel multiplex format of an extended multilocus variable-number-tandem-repeat analysis of Clostridium difficile correlates with tandem repeat sequence typing

J Microbiol Methods. 2015 Mar:110:98-101. doi: 10.1016/j.mimet.2015.01.018. Epub 2015 Jan 22.

Abstract

Subtyping of Clostridium difficile is crucial for outbreak investigations. An extended multilocus variable-number tandem-repeat analysis (eMLVA) of 14 variable number tandem repeat (VNTR) loci was validated in multiplex format compatible with a routine typing laboratory and showed excellent concordance with tandem repeat sequence typing (TRST) and high discriminatory power.

Keywords: Clostridium difficile; Molecular subtyping; Multilocus variable-number tandem-repeat analysis; Tandem repeat sequence typing.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Clostridioides difficile / classification*
  • Clostridioides difficile / genetics*
  • DNA, Bacterial
  • Minisatellite Repeats*
  • Multilocus Sequence Typing / methods*
  • Phylogeny
  • Ribotyping / methods*
  • Tandem Repeat Sequences*

Substances

  • DNA, Bacterial