Bioassay-guided identification of bioactive molecules from traditional Chinese medicines

Jianying Han; Jingyu Zhang; Wenni He; Pei Huang; Ayokunmi Oyeleye; Xueting Liu; Lixin Zhang

doi:10.1007/978-1-4939-2269-7_15

Bioassay-guided identification of bioactive molecules from traditional Chinese medicines

Methods Mol Biol. 2015:1263:187-96. doi: 10.1007/978-1-4939-2269-7_15.

Authors

Jianying Han¹, Jingyu Zhang, Wenni He, Pei Huang, Ayokunmi Oyeleye, Xueting Liu, Lixin Zhang

Affiliation

¹ Chinese Academy of Sciences Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.

PMID: 25618346
DOI: 10.1007/978-1-4939-2269-7_15

Abstract

Traditional Chinese medicines (TCMs) serve as a major source of a variety of drug lead compounds. In the process of natural products development, bioassay-guided isolation is a rapid and validated method for isolation of compounds with bioactivities. This chapter describes bioassay-guided separation and purification of compounds from the crude extracts of TCMs. Two approaches including size-exclusion chromatography (SEC) and high performance liquid chromatography (HPLC) are described in detail.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Infective Agents / chemistry
Anti-Infective Agents / isolation & purification
Anti-Infective Agents / pharmacology
Biological Products / chemistry
Biological Products / isolation & purification*
Biological Products / pharmacology*
Chromatography, Liquid*
Drugs, Chinese Herbal / chemistry
Drugs, Chinese Herbal / isolation & purification*
Drugs, Chinese Herbal / pharmacology*
Medicine, Chinese Traditional*
Microbial Sensitivity Tests / methods*

Substances

Anti-Infective Agents
Biological Products
Drugs, Chinese Herbal