High-protein diet induces oxidative stress in rat brain: protective action of high-intensity exercise against lipid peroxidation

Nutr Hosp. 2014 Oct 25;31(2):866-74. doi: 10.3305/nh.2015.31.2.8182.

Abstract
in English, Spanish

Introduction: It is well established that soy protein diets as well as aerobic exercise could promote antioxidant capacity and consequently reduce free radicals overproduction on brain. However, little is know regarding to the high-protein diets and high intensity exercise on oxidative stress production. The aim of this study was to analyse the effects of high-protein diets and high-intensity exercise (HIE) on brain oxidative stress markers.

Materials and methods: A total of 40 male Wistar rats were randomly distributed in 4 experimental groups (n=10): normal-protein or high-protein diets with or without HIE for an experimental period of 12 weeks. Main oxidative damage markers in brain such as thiobarbituric acid-reactive substances (TBARs) and protein carbonyl content (PCC) were assessed. In addition, brain manganese superoxide dismutase (Mn-SOD), cooper/ zinc superoxide dismutase (CuZn-SOD) and catalase (CAT) antioxidant enzymes activity, and protein level of Nuclear factor erythroid 2 related factor 2 (Nrf2) were measured.

Results and discussion: Brain TBARs, PCC, tSOD, Mn-SOD, CuZn-SOD and CAT levels were higher in the high-protein compared to the normal-protein groups (all, p.

Introducción: Es conocido que la proteína de soja así como la práctica de ejercicio físico aeróbico pueden incrementar la capacidad antioxidante y con ello reducir la sobreproducción de radicales libres en el cerebro. Sin embargo, existe desconocimiento sobre el efecto del consumo de dietas hiperproteicas y el entrenamiento de alta intensidad (EAI) sobre dicho estrés oxidativo. El objetivo del presente estudio fue analizar la influencia del consumo de una dieta hiperproteica y de EAI sobre marcadores de estrés oxidativo en cerebro. Métodos: Cuarenta ratas Wistar macho adultas fueron aleatoriamente distribuidas en 4 grupos experimentales (n=10): dieta normoproteica o hiperproteica, con o sin EAI durante un periodo experimental de 12 semanas. Se determinaron los principales marcadores de daño oxidativo en cerebro como sustancias reactivas del ácido tiobarbitúrico (TBARs) y el contenido de grupos carbonilos (PCC). Además, se midieron las actividades enzimáticas superóxido dismutasa del manganeso (Mn-SOD), de cobre/ zinc (CuZn-SOD) y catalasa (CAT), así como el nivel de proteína del factor nuclear eritroide-2 (Nrf2). Resultados: Los niveles de TBARs, PCC, tSOD, Mn- SOD, CuZn-SOD y CAT fueron significativamente mayores en los grupos hiperproteicos en comparación con los normoproteicos (todas, p.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antioxidants / metabolism
  • Brain Chemistry / physiology*
  • Diet
  • Dietary Proteins / pharmacology*
  • Lipid Peroxidation / physiology*
  • Male
  • Oxidative Stress*
  • Physical Conditioning, Animal / physiology*
  • Rats
  • Rats, Wistar

Substances

  • Antioxidants
  • Dietary Proteins