[Characterization of a cloned DNA fragment of rice chloroplast and location rbcL gene in the recombinant plasmid]

Abstract

The pure rice (Oryza sativa, subsp. Indica, cv. Zhenshan 97B) chloroplast DNA was digested by restriction enzyme BamHI and the resulting fragments were ligated to the BamHI site of pBR322. One recombinant plasmid which contains a 19.3kb insertel DNA fragment was isolated from the clone bank and was named pOSB1. A physical map of the recombinant plasmid was constructed by cleavage with ten restriction endonucleases, and the gene rbcL was located on the pOSB1.