Abstract
We report a novel strategy to enzymatically release affinity-selected cells, such as circulating tumor cells (CTCs), from surfaces with high efficiency (∼90%) while maintaining cell viability (>85%). The strategy utilizes single-stranded DNAs that link a capture antibody to the surfaces of a CTC selection device. The DNA linkers contain a uracil residue that can be cleaved.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Antibodies, Monoclonal / chemistry
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Antigens, CD34 / immunology
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Antigens, Neoplasm / immunology
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Cell Adhesion Molecules / immunology
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Cell Line, Tumor
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DNA, Single-Stranded / chemistry*
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Endodeoxyribonucleases / chemistry*
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Endopeptidases
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Epithelial Cell Adhesion Molecule
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Gelatinases / immunology
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Humans
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Membrane Proteins / immunology
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Neoplastic Cells, Circulating* / chemistry
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Serine Endopeptidases / immunology
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Uracil / chemistry*
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Uracil-DNA Glycosidase / chemistry*
Substances
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Antibodies, Monoclonal
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Antigens, CD34
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Antigens, Neoplasm
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Cell Adhesion Molecules
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DNA, Single-Stranded
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EPCAM protein, human
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Epithelial Cell Adhesion Molecule
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Membrane Proteins
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Uracil
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Endodeoxyribonucleases
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endodeoxyribonuclease VII
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Uracil-DNA Glycosidase
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Endopeptidases
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Serine Endopeptidases
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fibroblast activation protein alpha
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Gelatinases