Enzymatic cleavage of uracil-containing single-stranded DNA linkers for the efficient release of affinity-selected circulating tumor cells

Chem Commun (Camb). 2015 Feb 21;51(15):3266-9. doi: 10.1039/c4cc09765c.

Abstract

We report a novel strategy to enzymatically release affinity-selected cells, such as circulating tumor cells (CTCs), from surfaces with high efficiency (∼90%) while maintaining cell viability (>85%). The strategy utilizes single-stranded DNAs that link a capture antibody to the surfaces of a CTC selection device. The DNA linkers contain a uracil residue that can be cleaved.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal / chemistry
  • Antigens, CD34 / immunology
  • Antigens, Neoplasm / immunology
  • Cell Adhesion Molecules / immunology
  • Cell Line, Tumor
  • DNA, Single-Stranded / chemistry*
  • Endodeoxyribonucleases / chemistry*
  • Endopeptidases
  • Epithelial Cell Adhesion Molecule
  • Gelatinases / immunology
  • Humans
  • Membrane Proteins / immunology
  • Neoplastic Cells, Circulating* / chemistry
  • Serine Endopeptidases / immunology
  • Uracil / chemistry*
  • Uracil-DNA Glycosidase / chemistry*

Substances

  • Antibodies, Monoclonal
  • Antigens, CD34
  • Antigens, Neoplasm
  • Cell Adhesion Molecules
  • DNA, Single-Stranded
  • EPCAM protein, human
  • Epithelial Cell Adhesion Molecule
  • Membrane Proteins
  • Uracil
  • Endodeoxyribonucleases
  • endodeoxyribonuclease VII
  • Uracil-DNA Glycosidase
  • Endopeptidases
  • Serine Endopeptidases
  • fibroblast activation protein alpha
  • Gelatinases