Abstract
3-Hydroxypropionic acid (3-HP) is an important platform chemical for the biobased chemical industry. Lactobacillus reuteri produces 3-HP from glycerol via 3-hydroxypropionaldehyde (3-HPA) through a CoA-dependent propanediol utilization (Pdu) pathway. This study was performed to verify and evaluate the pathway comprising propionaldehyde dehydrogenase (PduP), phosphotransacylase (PduL), and propionate kinase (PduW) for formation of 3-HP from 3-HPA. The pathway was confirmed using recombinant Escherichia coli co-expressing PduP, PduL and PduW of L. reuteri DSM 20016 and mutants lacking expression of either enzyme. Growing and resting cells of the recombinant strain produced 3-HP with a yield of 0.3mol/mol and 1mol/mol, respectively, from 3-HPA. 3-HP was the sole product with resting cells, while growing cells produced 1,3-propanediol as co-product. 3-HP production from glycerol was achieved with a yield of 0.68mol/mol by feeding recombinant E. coli with 3-HPA produced by L. reuteri and recovered using bisulfite-functionalized resin.
Keywords:
3-Hydroxypropionic acid; Lactobacillus reuteri; Propanediol utilization pathway; Recombinant Escherichia coli; Two-step biotransformation.
Copyright © 2015 Elsevier Ltd. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aldehyde Oxidoreductases / genetics
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Aldehyde Oxidoreductases / metabolism
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Aldehyde Reductase / genetics
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Base Sequence
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Cloning, Molecular
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Escherichia coli / genetics*
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Escherichia coli / metabolism
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Escherichia coli Proteins / genetics
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Escherichia coli Proteins / metabolism
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Glyceraldehyde / analogs & derivatives*
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Glyceraldehyde / metabolism
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Glycerol / metabolism
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Lactic Acid / analogs & derivatives*
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Lactic Acid / biosynthesis
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Lactic Acid / metabolism
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Limosilactobacillus reuteri / genetics
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Limosilactobacillus reuteri / metabolism*
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Molecular Sequence Data
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Organisms, Genetically Modified
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Phosphate Acetyltransferase / genetics
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Phosphate Acetyltransferase / metabolism
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Phosphotransferases (Carboxyl Group Acceptor) / genetics
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Phosphotransferases (Carboxyl Group Acceptor) / metabolism
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Propane / metabolism*
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism*
Substances
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Escherichia coli Proteins
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Recombinant Proteins
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3-hydroxypropionaldehyde
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Lactic Acid
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Glyceraldehyde
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hydracrylic acid
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Aldehyde Reductase
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YqhD protein, E coli
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Aldehyde Oxidoreductases
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coenzyme A-acylating propionaldehyde dehydrogenase, Lactobacillus reuteri
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Phosphate Acetyltransferase
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Phosphotransferases (Carboxyl Group Acceptor)
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TdcD protein, E coli
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Glycerol
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Propane