A pilot evaluation of external quality assessment of GenoType MTBDRplus versions 1 and 2 using dried culture spot material

N Gous; L E Isherwood; A David; W Stevens; L E Scott

doi:10.1128/JCM.03340-14

A pilot evaluation of external quality assessment of GenoType MTBDRplus versions 1 and 2 using dried culture spot material

J Clin Microbiol. 2015 Apr;53(4):1365-7. doi: 10.1128/JCM.03340-14. Epub 2015 Jan 21.

Authors

N Gous¹, L E Isherwood², A David², W Stevens³, L E Scott⁴

Affiliations

¹ Department of Molecular Medicine and Haematology, School of Pathology, University of the Witwatersrand, Johannesburg, South Africa natasha.gous@gmail.com.
² National Health Laboratory Service, Johannesburg, South Africa.
³ Department of Molecular Medicine and Haematology, School of Pathology, University of the Witwatersrand, Johannesburg, South Africa National Health Laboratory Service, Johannesburg, South Africa.
⁴ Department of Molecular Medicine and Haematology, School of Pathology, University of the Witwatersrand, Johannesburg, South Africa.

Abstract

Dried culture spots (DCS) of inactivated Mycobacteria strains designed as part of an external quality assessment (EQA) program for the GeneXpert system has applications to other molecular tuberculosis (TB) diagnostic platforms. DCS tested on the GenoType MTBDRplus and Mycobacterium CM assays performed well with MTBDRplus version 2 but require increased bacterial concentration for use with version 1.

MeSH terms

Bacteriological Techniques / methods*
Bacteriological Techniques / standards
Genotype
Humans
Molecular Diagnostic Techniques
Mycobacterium / genetics*
Mycobacterium / isolation & purification*
Pilot Projects
Sensitivity and Specificity
Species Specificity