Cell type-specific gene expression profiling in brain tissue: comparison between TRAP, LCM and RNA-seq

TaeHyun Kim; Chae-Seok Lim; Bong-Kiun Kaang

doi:10.5483/bmbrep.2015.48.7.218

Cell type-specific gene expression profiling in brain tissue: comparison between TRAP, LCM and RNA-seq

BMB Rep. 2015 Jul;48(7):388-94. doi: 10.5483/bmbrep.2015.48.7.218.

Authors

TaeHyun Kim¹, Chae-Seok Lim¹, Bong-Kiun Kaang¹

Affiliation

¹ Department of Biological Sciences, Seoul National University, Seoul 151-747, Korea.

Abstract

The brain is an organ that consists of various cell types. As our knowledge of the structure and function of the brain progresses, cell type-specific research is gaining importance. Together with advances in sequencing technology and bioinformatics, cell type-specific transcriptome studies are providing important insights into brain cell function. In this review, we discuss 3 different cell type-specific transcriptome analyses i.e., Laser Capture Microdissection (LCM), Translating Ribosome Affinity Purification (TRAP)/RiboTag, and single cell RNA-Seq, that are widely used in the field of neuroscience.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Brain / metabolism*
Chromatography, Affinity / methods*
Gene Expression Profiling / methods*
Humans
Laser Capture Microdissection / methods*
Organ Specificity / genetics*
Sequence Analysis, RNA / methods*