Complexed soluble IL-7 receptor α and IL-7 increase IL-7-mediated proliferation and viability of CD8⁺ T-cells in vitro

Sandra Côté; Julie Matte; Subash Sad; Jonathan B Angel; Angela M Crawley

doi:10.1016/j.cellimm.2015.01.001

Complexed soluble IL-7 receptor α and IL-7 increase IL-7-mediated proliferation and viability of CD8⁺ T-cells in vitro

Cell Immunol. 2015 Feb;293(2):122-5. doi: 10.1016/j.cellimm.2015.01.001. Epub 2015 Jan 10.

Authors

Sandra Côté¹, Julie Matte¹, Subash Sad², Jonathan B Angel³, Angela M Crawley⁴

Affiliations

¹ Ottawa Hospital Research Institute, Chronic Disease Program, Ottawa, ON, Canada; University of Ottawa, Department of Biochemistry, Microbiology and Immunology, Ottawa, ON, Canada.
² University of Ottawa, Department of Biochemistry, Microbiology and Immunology, Ottawa, ON, Canada.
³ Ottawa Hospital Research Institute, Chronic Disease Program, Ottawa, ON, Canada; University of Ottawa, Department of Biochemistry, Microbiology and Immunology, Ottawa, ON, Canada; Ottawa Hospital, Division of Infectious Diseases, Ottawa, ON, Canada.
⁴ Ottawa Hospital Research Institute, Chronic Disease Program, Ottawa, ON, Canada; University of Ottawa, Department of Biochemistry, Microbiology and Immunology, Ottawa, ON, Canada. Electronic address: acrawley@ohri.ca.

PMID: 25596474
DOI: 10.1016/j.cellimm.2015.01.001

Abstract

Many soluble cytokine receptors inhibit cytokine bioactivity, while others prolong ligand activity. The biological role of an endogenous soluble form of IL-7Rα, or its therapeutic effects on CD8(+) T-cells are unknown. We demonstrate that recombinant IL-7Rα-Fc, when pre-incubated with IL-7, enhances IL-7-induced CD8(+) T-cell proliferation and viability of human or murine CD8(+) T-cells. Receptor blocking experiments confirmed IL-7-specific activity. These data demonstrate that exogenous soluble IL-7Rα significantly enhances CD8(+) T-cell responses to IL-7 in vitro and paves the way for future research to determine its therapeutic potential to restore impaired CD8(+) T-cell function in disease.

Keywords: CD8(+) T-cells; Cell proliferation; Human; IL-7 receptor; Murine.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
CD8-Positive T-Lymphocytes / immunology*
Cell Proliferation / drug effects*
Humans
Interleukin-7 / immunology*
Mice
Mice, Inbred C57BL
Receptors, Interleukin-7 / immunology*

Substances

Interleukin-7
Receptors, Interleukin-7
interleukin-7 receptor, alpha chain

Grants and funding

Canadian Institutes of Health Research/Canada