Insulin and IGF-I receptors in neuroblastoma cells: increases in mRNA and binding produced by glyburide

Neuropeptides. 1989 Oct;14(3):171-5. doi: 10.1016/0143-4179(89)90041-3.

Abstract

Insulin and IGF-I binding to neuroblastoma cells (SK-N-MC) was increased by 13% and 7% respectively following a 24hr, incubation with the sulphonylurea glyburide. This increase in binding was associated with increased steady-state levels of insulin receptor and IGF-I receptor mRNA levels. Though insulin and IGF-I both stimulate glucose uptake into these cells, the increased binding following glyburide treatment was not associated with any change in glucose uptake.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Deoxyglucose / metabolism
  • Glyburide / pharmacology
  • Humans
  • Insulin-Like Growth Factor I / metabolism
  • Neuroblastoma / metabolism
  • RNA, Messenger / metabolism
  • RNA, Neoplasm / metabolism
  • Receptor, Insulin / drug effects
  • Receptor, Insulin / metabolism*
  • Receptors, Cell Surface / drug effects
  • Receptors, Cell Surface / metabolism*
  • Receptors, Somatomedin
  • Tumor Cells, Cultured / drug effects
  • Tumor Cells, Cultured / metabolism*

Substances

  • RNA, Messenger
  • RNA, Neoplasm
  • Receptors, Cell Surface
  • Receptors, Somatomedin
  • Insulin-Like Growth Factor I
  • Deoxyglucose
  • Receptor, Insulin
  • Glyburide