Abstract
Insulin and IGF-I binding to neuroblastoma cells (SK-N-MC) was increased by 13% and 7% respectively following a 24hr, incubation with the sulphonylurea glyburide. This increase in binding was associated with increased steady-state levels of insulin receptor and IGF-I receptor mRNA levels. Though insulin and IGF-I both stimulate glucose uptake into these cells, the increased binding following glyburide treatment was not associated with any change in glucose uptake.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Deoxyglucose / metabolism
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Glyburide / pharmacology
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Humans
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Insulin-Like Growth Factor I / metabolism
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Neuroblastoma / metabolism
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RNA, Messenger / metabolism
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RNA, Neoplasm / metabolism
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Receptor, Insulin / drug effects
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Receptor, Insulin / metabolism*
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Receptors, Cell Surface / drug effects
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Receptors, Cell Surface / metabolism*
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Receptors, Somatomedin
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Tumor Cells, Cultured / drug effects
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Tumor Cells, Cultured / metabolism*
Substances
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RNA, Messenger
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RNA, Neoplasm
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Receptors, Cell Surface
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Receptors, Somatomedin
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Insulin-Like Growth Factor I
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Deoxyglucose
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Receptor, Insulin
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Glyburide