Isolated human bladder: evidence for an adenine dinucleotide acting on P2X-purinoceptors and for purinergic transmission

Eur J Pharmacol. 1989 Dec 12;174(1):115-8. doi: 10.1016/0014-2999(89)90881-9.

Abstract

In isolated strips of human urinary bladder detrusor muscle, ATP, alpha, beta-methylene ATP and P1,P6-diadenosine hexaphosphate caused concentration-dependent contractions. ATP was less potent than the two synthetic purine compounds and gave smaller maximum responses. Responses to ATP, P1,P6-diadenosine hexaphosphate and noncholinergic nerve stimulation were blocked following desensitization of P2X-purinoceptors by alpha,beta-methylene ATP. Thus, adenine dinucleotides can act on P2X-purinoceptors and there is an element of purinergic neuromuscular transmission in the human urinary bladder.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenine Nucleotides / pharmacology*
  • Adenosine Triphosphate / analogs & derivatives
  • Adenosine Triphosphate / pharmacology
  • Adult
  • Aged
  • Dinucleoside Phosphates / pharmacology
  • Electric Stimulation
  • Female
  • Humans
  • In Vitro Techniques
  • Male
  • Middle Aged
  • Muscle, Smooth / drug effects*
  • Neuromuscular Junction / drug effects
  • Receptors, Purinergic / drug effects*
  • Synaptic Transmission / drug effects
  • Urinary Bladder / drug effects

Substances

  • Adenine Nucleotides
  • Dinucleoside Phosphates
  • Receptors, Purinergic
  • P(1),P(5)-di(adenosine-5'-)pentaphosphate
  • Adenosine Triphosphate
  • alpha,beta-methyleneadenosine 5'-triphosphate