Simultaneous determination of levodopa, carbidopa, entacapone, tolcapone, 3-O-methyldopa and dopamine in human plasma by an HPLC-MS/MS method

Bioanalysis. 2015;7(2):207-20. doi: 10.4155/bio.14.230.

Abstract

Background: In this study, we developed and validated a HPLC-MS/MS method capable of simultaneously determining levodopa, carbidopa, entacapone, tolcapone, 3-O-methyldopa and dopamine in human plasma. RESULTS & METHODOLOGY: Chromatographic separation was achieved using a C8 column with a mobile phase consisting of a gradient of water and acetonitrile:methanol (90:10 v/v), both containing 0.1% formic acid. The developed method was selective, sensitive (LD<7.0 ng ml(-1)), linear (r>0.99), precise (RSD<11.3%), accurate (RE<11.8%) and free of residual and matrix effects. The developed method was successfully applied in plasma patients with Parkinson's disease using Stalevo®.

Conclusion: The new method can be used for the clinical monitoring of these substances and applied to adjustments in drug dosages.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Benzophenones / blood*
  • Benzophenones / standards
  • Blood Chemical Analysis / methods*
  • Carbidopa / blood*
  • Carbidopa / standards
  • Catechols / blood*
  • Catechols / standards
  • Chromatography, High Pressure Liquid* / standards
  • Dihydroxyphenylalanine / analogs & derivatives*
  • Dihydroxyphenylalanine / blood
  • Dihydroxyphenylalanine / standards
  • Dopamine / blood*
  • Dopamine / standards
  • Humans
  • Levodopa / blood*
  • Levodopa / standards
  • Nitriles / blood*
  • Nitriles / standards
  • Nitrophenols / blood*
  • Nitrophenols / standards
  • Quality Control
  • Reproducibility of Results
  • Tandem Mass Spectrometry* / standards
  • Tolcapone
  • Tyrosine / analogs & derivatives

Substances

  • Benzophenones
  • Catechols
  • Nitriles
  • Nitrophenols
  • Tyrosine
  • Levodopa
  • entacapone
  • Dihydroxyphenylalanine
  • Tolcapone
  • Carbidopa
  • 3-methoxytyrosine
  • Dopamine