Abstract
Background:
In this study, we developed and validated a HPLC-MS/MS method capable of simultaneously determining levodopa, carbidopa, entacapone, tolcapone, 3-O-methyldopa and dopamine in human plasma. RESULTS & METHODOLOGY: Chromatographic separation was achieved using a C8 column with a mobile phase consisting of a gradient of water and acetonitrile:methanol (90:10 v/v), both containing 0.1% formic acid. The developed method was selective, sensitive (LD<7.0 ng ml(-1)), linear (r>0.99), precise (RSD<11.3%), accurate (RE<11.8%) and free of residual and matrix effects. The developed method was successfully applied in plasma patients with Parkinson's disease using Stalevo®.
Conclusion:
The new method can be used for the clinical monitoring of these substances and applied to adjustments in drug dosages.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Benzophenones / blood*
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Benzophenones / standards
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Blood Chemical Analysis / methods*
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Carbidopa / blood*
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Carbidopa / standards
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Catechols / blood*
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Catechols / standards
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Chromatography, High Pressure Liquid* / standards
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Dihydroxyphenylalanine / analogs & derivatives*
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Dihydroxyphenylalanine / blood
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Dihydroxyphenylalanine / standards
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Dopamine / blood*
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Dopamine / standards
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Humans
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Levodopa / blood*
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Levodopa / standards
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Nitriles / blood*
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Nitriles / standards
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Nitrophenols / blood*
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Nitrophenols / standards
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Quality Control
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Reproducibility of Results
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Tandem Mass Spectrometry* / standards
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Tolcapone
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Tyrosine / analogs & derivatives
Substances
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Benzophenones
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Catechols
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Nitriles
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Nitrophenols
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Tyrosine
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Levodopa
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entacapone
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Dihydroxyphenylalanine
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Tolcapone
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Carbidopa
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3-methoxytyrosine
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Dopamine