Scrutinizing calcium flux oscillations in T lymphocytes to deduce the strength of stimulus

Susan N Christo; Kerrilyn R Diener; Robert E Nordon; Michael P Brown; Hans J Griesser; Krasimir Vasilev; Farid C Christo; John D Hayball

doi:10.1038/srep07760

Scrutinizing calcium flux oscillations in T lymphocytes to deduce the strength of stimulus

Sci Rep. 2015 Jan 14:5:7760. doi: 10.1038/srep07760.

Authors

Susan N Christo^{1

2}, Kerrilyn R Diener^{1

2

3}, Robert E Nordon⁴, Michael P Brown^{1

2

5

6}, Hans J Griesser⁷, Krasimir Vasilev⁸, Farid C Christo⁹, John D Hayball^{1

2

6}

Affiliations

¹ Experimental Therapeutics Laboratory, Hanson Institute, Adelaide, SA 5000, Australia.
² Sansom Institute and School of Pharmacy and Medical Sciences, University of South Australia, Adelaide, SA 5000, Australia.
³ Robinson Research Institute and School of Paediatrics and Reproductive Health, University of Adelaide, Adelaide, SA 5005, Australia.
⁴ Graduate School of Biomedical Engineering, University of New South Wales, Kensington, NSW, 2052, Australia.
⁵ Cancer Clinical Trials Unit, Royal Adelaide Hospital, Adelaide, SA 5000, Australia.
⁶ School of Medicine, University of Adelaide, Adelaide, SA 5005, Australia.
⁷ Ian Wark Research Institute, University of South Australia, Adelaide, SA 5095, Australia.
⁸ Mawson Institute, University of South Australia, Adelaide, SA 5000, Australia.
⁹ Barbara Hardy Institute, University of South Australia, Adelaide, SA 5000, Australia.

Abstract

The capture and activation of individual T cells on functionalised surfaces enables real-time analyses of the magnitude and rhythm of intracellular calcium release. Application of Haarlet transformations generate a calcium flux 'threshold', with the frequency of the 'threshold crossings' correlating with the strength of the original T cell stimulus. These findings represent a new method to evaluate graduations in T cell activation in real time, and at a single-cell level.

MeSH terms

Animals
CD28 Antigens / metabolism
CD3 Complex / metabolism
Calcium Signaling*
Fluorescence
Ligands
Lymphocyte Activation / immunology*
Mice, Inbred C57BL
Receptors, Antigen, T-Cell / metabolism
T-Lymphocytes / metabolism*

Substances

CD28 Antigens
CD3 Complex
Ligands
Receptors, Antigen, T-Cell