LH signaling is required for oocyte maturation in fish and other vertebrates. However, the downstream factors mediating LH signaling are largely unexplored in fish. In this study, we investigated whether IGFs could mediate LH action on oocyte maturation in zebrafish. Our results show that all igfs, including igf1, igf2a, igf2b, and igf3, are dynamically expressed during folliculogenesis, with the expression of igf3 reaching its maximal level in full grown stage follicles. The expression of igfs is regulated by LH through a cAMP pathway in intact follicles as well as in primary cultured follicular cells, with igf3 expression being the most sensitive to human chorionic gonadotropin (hCG) treatment. Moreover, recombinant zebrafish IGF-2a, IGF-2b, and IGF-3 proteins significantly enhanced oocyte maturation via IGF-1 receptors (IGF-1rs), with IGF-3 exhibiting the most potent stimulatory action on oocyte maturation. Furthermore, we have demonstrated that IGF-3 or hCG treatment could stimulate IGF-1rs phosphorylation, and hCG-induced oocyte maturation could be attenuated by IGF-1r inhibitors as well as by an anti-IGF-3 antiserum in vitro and in vivo, indicating that the IGF system especially IGF-3 plays a crucial role in mediating LH action on oocyte maturation. In addition, igf3 expression is significantly attenuated in LH β-subunit (lhb) mutant zebrafish and treatment with recombinant IGF-3 could partially rescue the oocyte maturation defects of the lhb mutants in vitro and in vivo. Collectively, our results clearly demonstrated that IGFs, particularly the gonad-specific IGF-3, act as important mediators of LH action on oocyte maturation in zebrafish.