Periapical cytokine expression in sickle cell disease

Shirlene Barbosa Pimentel Ferreira; Luciana Carla Neves de Brito; Michelle Pimenta Oliveira; Kamilla Faria Maciel; Hercílio Martelli Júnior; Leda Quercia Vieira; Antônio Paulino Ribeiro Sobrinho

doi:10.1016/j.joen.2014.11.016

Periapical cytokine expression in sickle cell disease

J Endod. 2015 Mar;41(3):358-62. doi: 10.1016/j.joen.2014.11.016. Epub 2015 Jan 6.

Authors

Shirlene Barbosa Pimentel Ferreira¹, Luciana Carla Neves de Brito², Michelle Pimenta Oliveira³, Kamilla Faria Maciel⁴, Hercílio Martelli Júnior³, Leda Quercia Vieira⁴, Antônio Paulino Ribeiro Sobrinho⁵

Affiliations

¹ Departamento de Odontologia Restauradora, Faculdade de Odontologia, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte.
² Faculdade de Odontologia, Fundação Universidade de Itaúna (FUI), Itaúna.
³ Faculdade de Odontologia de Montes Claros, Universidade Estadual de Minas Gerais (UNIMONTES), Montes Claros.
⁴ Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Minas Gerais, Brazil.
⁵ Departamento de Odontologia Restauradora, Faculdade de Odontologia, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte. Electronic address: sobrinho.bhz@terra.com.br.

PMID: 25576201
DOI: 10.1016/j.joen.2014.11.016

Abstract

Introduction: Sickle cell anemia (SCA) is the most prevalent genetic disease worldwide. Patients with SCA exhibit increased levels of proinflammatory mediators as part of a permanently activated immunoinflammatory status.

Methods: The aim of this study was to evaluate the mRNA expression levels of the cytokines interferon (IFN-γ), tumor necrosis factor, interleukin (IL-1β, IL-17A, IL-10), receptor activator for nuclear factor kappa B ligand, and the chemokines CCL2/MCP-1 and CCL5 in the periapical interstitial fluid from SCA individuals compared with healthy individuals. Samples were collected from 12 teeth of SCA patients and 12 non-SCA patients with apical periodontitis. In addition, 12 teeth were sampled from the periapical region of healthy patients with vital pulp (control). The expression of cytokine mRNA was detected by using real-time polymerase chain reaction.

Results: The expression of mRNA for the Th1-associated cytokines IFN-γ, tumor necrosis factor-α, and IL-1β were significantly higher in SCA individuals than in the control individuals (P < .05). Among Th1-associated cytokines, only IFN-γ was significantly increased in non-SCA compared with control patients (vital pulp). The expression of IL-17A mRNA was significant higher in SCA cases than in control samples (P < .05), whereas the IL-10 mRNA expression was significantly increased in SCA and non-SCA individuals when compared with the control group. Similar levels of receptor activator for nuclear factor kappa B ligand, CCL2, and CCL5 mRNA expression were observed in all samples. However, no significant differences were observed in the expression of cytokine or chemokine mRNA between SCA and non-SCA individuals (P > .05).

Conclusions: The results were able to demonstrate that SCA patients presented prone proinflammatory ability, despite the fact that any differences in periapical immune responses between SCA and non-SCA individuals were not observed.

Keywords: Chemokine; cytokine; periapical interstitial fluid; sickle cell anemia.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anemia, Sickle Cell / genetics
Anemia, Sickle Cell / metabolism*
Case-Control Studies
Cytokines / genetics
Cytokines / metabolism*
Gene Expression Regulation
Humans
Periapical Tissue / metabolism*

Substances

Cytokines