A high-throughput screening assay for identification of inhibitors of the A1AO-ATP synthase of the rumen methanogen Methanobrevibacter ruminantium M1

Htin Lin Aung; Debjit Dey; Peter H Janssen; Ron S Ronimus; Gregory M Cook

doi:10.1016/j.mimet.2014.12.022

A high-throughput screening assay for identification of inhibitors of the A1AO-ATP synthase of the rumen methanogen Methanobrevibacter ruminantium M1

J Microbiol Methods. 2015 Mar:110:15-7. doi: 10.1016/j.mimet.2014.12.022. Epub 2015 Jan 7.

Authors

Htin Lin Aung¹, Debjit Dey², Peter H Janssen², Ron S Ronimus², Gregory M Cook³

Affiliations

¹ Department of Microbiology and Immunology, Otago School of Medical Sciences, University of Otago, Dunedin, New Zealand.
² AgResearch Limited, Grasslands Research Centre, Palmerston North, New Zealand.
³ Department of Microbiology and Immunology, Otago School of Medical Sciences, University of Otago, Dunedin, New Zealand. Electronic address: gregory.cook@otago.ac.nz.

PMID: 25575416
DOI: 10.1016/j.mimet.2014.12.022

Abstract

We report the development of a high-throughput screening platform to identify inhibitors of the membrane-bound A1Ao-ATP synthase from the rumen methanogen Methanobrevibacter ruminantium M1. Inhibitors identified in the screen were tested against growing cultures of M. ruminantium, validating the approach to identify new inhibitors of methanogens.

Keywords: A(1)A(O)-ATP synthase; High-throughput screening; Inhibitor; Methane; Methanobrevibacter ruminantium.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adenosine Triphosphate / biosynthesis*
Adenosine Triphosphate / metabolism
Animals
Enzyme Assays / methods*
High-Throughput Screening Assays*
Methanobrevibacter / enzymology*
Methanobrevibacter / genetics
Methanobrevibacter / growth & development
Phylogeny
Proton-Translocating ATPases / antagonists & inhibitors*
Rumen / microbiology*

Substances

Adenosine Triphosphate
Proton-Translocating ATPases