Structural basis for preferential avian receptor binding by the human-infecting H10N8 avian influenza virus

Min Wang; Wei Zhang; Jianxun Qi; Fei Wang; Jianfang Zhou; Yuhai Bi; Ying Wu; Honglei Sun; Jinhua Liu; Chaobin Huang; Xiangdong Li; Jinghua Yan; Yuelong Shu; Yi Shi; George F Gao

doi:10.1038/ncomms6600

Structural basis for preferential avian receptor binding by the human-infecting H10N8 avian influenza virus

Nat Commun. 2015 Jan 9:6:5600. doi: 10.1038/ncomms6600.

Authors

Min Wang¹, Wei Zhang², Jianxun Qi², Fei Wang¹, Jianfang Zhou³, Yuhai Bi², Ying Wu², Honglei Sun⁴, Jinhua Liu⁴, Chaobin Huang⁵, Xiangdong Li⁵, Jinghua Yan⁴, Yuelong Shu³, Yi Shi⁶, George F Gao⁷

Affiliations

¹ 1] College of Veterinary Medicine, China Agricultural University, Beijing 100193, China [2] CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
² CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
³ National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention (China CDC), Beijing 102206, China.
⁴ College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
⁵ State Key Laboratory of Agro-biotechnology, China Agricultural University, Beijing 100193, China.
⁶ 1] CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China [2] Research Network of Immunity and Health (RNIH), Beijing Institutes of Life Science, Chinese Academy of Sciences, Beijing 100101, China.
⁷ 1] College of Veterinary Medicine, China Agricultural University, Beijing 100193, China [2] CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China [3] National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention (China CDC), Beijing 102206, China [4] Research Network of Immunity and Health (RNIH), Beijing Institutes of Life Science, Chinese Academy of Sciences, Beijing 100101, China [5] Office of Director-General, Chinese Center for Disease Control and Prevention (China CDC), Beijing 102206, China.

PMID: 25574798
DOI: 10.1038/ncomms6600

Abstract

Since December 2013, at least three cases of human infections with H10N8 avian influenza virus have been reported in China, two of them being fatal. To investigate the epidemic potential of H10N8 viruses, we examined the receptor binding property of the first human isolate, A/Jiangxi-Donghu/346/2013 (JD-H10N8), and determined the structures of its haemagglutinin (HA) in complex with both avian and human receptor analogues. Our results suggest that JD-H10N8 preferentially binds the avian receptor and that residue R137-localized within the receptor-binding site of HA-plays a key role in this preferential binding. Compared with the H7N9 avian influenza viruses, JD-H10N8 did not exhibit the enhanced binding to human receptors observed with the prevalent H7N9 virus isolate Anhui-1, but resembled the receptor binding activity of the early-outbreak H7N9 isolate (Shanghai-1). We conclude that the H10N8 virus is a typical avian influenza virus.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
China
Crystallography, X-Ray
Ducks
Hemagglutinin Glycoproteins, Influenza Virus / chemistry*
Humans
Influenza A Virus, H10N8 Subtype / chemistry*
Influenza A Virus, H7N9 Subtype / chemistry
Influenza in Birds / virology
Influenza, Human / immunology
Influenza, Human / virology*
Intestines / virology
Molecular Sequence Data
Protein Binding
Protein Structure, Secondary
Protein Structure, Tertiary
Receptors, Virus / chemistry*
Recombinant Proteins / chemistry
Surface Plasmon Resonance
Trachea / virology

Substances

Hemagglutinin Glycoproteins, Influenza Virus
Receptors, Virus
Recombinant Proteins