Role of supernumerary subunits in mitochondrial cytochrome c oxidase

Y Planques; N Capitanio; G Capitanio; E De Nitto; G Villani; S Papa

doi:10.1016/0014-5793(89)81675-8

Role of supernumerary subunits in mitochondrial cytochrome c oxidase

FEBS Lett. 1989 Dec 4;258(2):285-8. doi: 10.1016/0014-5793(89)81675-8.

Authors

Y Planques¹, N Capitanio, G Capitanio, E De Nitto, G Villani, S Papa

Affiliation

¹ Institute of Medical Biochemistry and Chemistry, University of Bari, Italy.

PMID: 2557239
DOI: 10.1016/0014-5793(89)81675-8

Abstract

Role of supernumerary subunits of bovine heart cytochrome c oxidase has been investigated by examining the influence on the enzymatic activity of their removal by chromatographic procedures or controlled digestion by trypsin. Is has been shown that partial proteolytic cleavage of subunit IV results in depression of respiratory activity and of redox-linked proton translocation. Selective removal by gel-filtration of subunit Vlb has no significant influence on the redox and protonmotive activity of the oxidase.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cattle
Cytochrome a Group
Cytochromes / metabolism
Electron Transport Complex IV / isolation & purification
Electron Transport Complex IV / metabolism*
Electrophoresis, Polyacrylamide Gel
Kinetics
Macromolecular Substances
Mitochondria, Heart / enzymology*
Peptide Fragments / isolation & purification
Trypsin / metabolism

Substances

Cytochrome a Group
Cytochromes
Macromolecular Substances
Peptide Fragments
Electron Transport Complex IV
Trypsin