Owing to interacting with the living tissue directly, the electrode-tissue interface largely determines the performance of the whole bioelectronics devices. The miniaturization of biomedical electronic components requires interface materials to possess properties including excellent electrical performance, good biocompatibility and compatibility with microelectronic fabrication process. Considering the unique characteristics and wide applications in biomedical domain of conducting polymer and graphene, composite film consists of poly(3,4-ethylenedioxythiophene) (PEDOT) and graphene oxide (GO) is proposed as electrode-tissue interface in this work. The facilely electrochemically synthesized PEDOT/GO coating on microelectrodes shows low impedance, high charge storage capacity and good biocompatibility to act as electrode-tissue interface. As a result, the composite film is a potential biomaterial as electrode-tissue interface for tissue engineering and further implantable electrophysiological devices.