Objective: To establish the rapid tissue propagation system of Lonicera macranthoides 'Yuleil ', in order to provide theoretical basis for industrialized seed cultivation.
Methods: Young stem and leaves of Lonicera macranthoides 'Yuleil' were used as explants, with MS as basic media, and added with different concentrations of plant growth regulators such as NAA, 6-BA, TDZ and IBA in different stages of tissue culture, in order to conduct a systematic study on callus induction, proliferation and differentiation, and stem axillary bud induction, proliferation, rooting and seedlings.
Results: Best sterilization time of explant was:stem for 2 - 3 min, leaves for 1 - 2 min. The optimal medium for callus induction was MS + 2,4-D 1.0 mg/L + 6-BA 0.1 mg/L. The optimal medium for axillary bud induction, callus proliferation, callus differentiation, bud proliferation and rooting was MS + 6-BA 1.0 mg/L + NAA 0.5 mg/L, MS + TDZ (0.2 - 1.0) mg/L + NAA (0.02 - 0.05) mg/L, MS + 6-BA (0.5 - 1.0) mg/L + NAA (0.02 - 0.05) mg/L, MS + 6-BA 1.5 mg/L + NAA 0.5 mg/L and 1/2MS + IBA 1.0 mg/L + NAA 0.2 mg/L, respectively. Tube seedlings was refined in matrix include pearlite and humus (1: 1), with the survival rate of more than 75%.
Conclusion: The disinfection method suitable for explants and the medium combination suitable for callus induction, proliferation, differentiation as well as bud proliferation and rooting are screened out to establish the rapid cultivation system for Lonicera macranthoides 'Yuleil'.