NeuO: a fluorescent chemical probe for live neuron labeling

Angew Chem Int Ed Engl. 2015 Feb 16;54(8):2442-6. doi: 10.1002/anie.201408614. Epub 2015 Jan 7.

Abstract

To address existing limitations in live neuron imaging, we have developed NeuO, a novel cell-permeable fluorescent probe with an unprecedented ability to label and image live neurons selectively over other cells in the brain. NeuO enables robust live neuron imaging and isolation in vivo and in vitro across species; its versatility and ease of use sets the basis for its development in a myriad of neuronal targeting applications.

Keywords: BODIPY; fluorescent probes; imaging agents; live neurons.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Boron Compounds / chemistry
  • Boron Compounds / metabolism
  • Cells, Cultured
  • Fluorescent Dyes / chemistry
  • Fluorescent Dyes / metabolism*
  • Microscopy, Confocal
  • Microscopy, Video
  • Neurons / cytology
  • Neurons / metabolism*
  • Rats
  • Staining and Labeling

Substances

  • 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene
  • Boron Compounds
  • Fluorescent Dyes