Coumarin 545: an emission reference dye with a record-low temperature coefficient for ratiometric fluorescence based temperature measurements

Deqi Mao; Xiaogang Liu; Qinglong Qiao; Wenting Yin; Miao Zhao; Jacqueline M Cole; Jingnan Cui; Zhaochao Xu

doi:10.1039/c4an02075h

Coumarin 545: an emission reference dye with a record-low temperature coefficient for ratiometric fluorescence based temperature measurements

Analyst. 2015 Feb 21;140(4):1008-13. doi: 10.1039/c4an02075h.

Authors

Deqi Mao¹, Xiaogang Liu, Qinglong Qiao, Wenting Yin, Miao Zhao, Jacqueline M Cole, Jingnan Cui, Zhaochao Xu

Affiliation

¹ State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116012, China. zcxu@dicp.ac.cn jncui@dlut.edu.cn.

PMID: 25563387
DOI: 10.1039/c4an02075h

Abstract

The emission intensities of coumarin 545 solution exhibit a low temperature dependence, with a record-low temperature coefficient of only ∼0.025% per °C. This monomer-aggregate coupled fluorescence system can be used for ratiometric temperature measurements with high spatial and temporal resolutions; three different working modes have been demonstrated.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Cold Temperature
Coumarins / chemistry*
Fluorescent Dyes / chemistry*
Spectrometry, Fluorescence
Temperature
Thermometers

Substances

Coumarins
Fluorescent Dyes
coumarin