Glucose transport machinery reconstituted in cell models

Chem Commun (Camb). 2015 Feb 11;51(12):2316-9. doi: 10.1039/c4cc08838g.

Abstract

Here we demonstrate the production of a functioning cell model by formation of giant vesicles reconstituted with the GLUT1 glucose transporter and a glucose oxidase and hydrogen peroxidase linked fluorescent reporter internally. Hence, a simplified artificial cell is formed that is able to take up glucose and process it.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biological Transport
  • Fluorescent Dyes / chemistry
  • Glucose / metabolism*
  • Glucose Oxidase / metabolism
  • Glucose Transporter Type 1 / metabolism*
  • Horseradish Peroxidase / chemistry
  • Phosphatidylcholines / chemistry
  • Unilamellar Liposomes / chemistry
  • Unilamellar Liposomes / metabolism

Substances

  • Fluorescent Dyes
  • Glucose Transporter Type 1
  • Phosphatidylcholines
  • Unilamellar Liposomes
  • 1,2-diphytanoylphosphatidylcholine
  • Glucose Oxidase
  • Horseradish Peroxidase
  • Glucose