Abstract
Here we demonstrate the production of a functioning cell model by formation of giant vesicles reconstituted with the GLUT1 glucose transporter and a glucose oxidase and hydrogen peroxidase linked fluorescent reporter internally. Hence, a simplified artificial cell is formed that is able to take up glucose and process it.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Biological Transport
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Fluorescent Dyes / chemistry
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Glucose / metabolism*
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Glucose Oxidase / metabolism
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Glucose Transporter Type 1 / metabolism*
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Horseradish Peroxidase / chemistry
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Phosphatidylcholines / chemistry
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Unilamellar Liposomes / chemistry
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Unilamellar Liposomes / metabolism
Substances
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Fluorescent Dyes
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Glucose Transporter Type 1
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Phosphatidylcholines
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Unilamellar Liposomes
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1,2-diphytanoylphosphatidylcholine
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Glucose Oxidase
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Horseradish Peroxidase
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Glucose