New emerging bio-catalysts design in biotransformations

Jose M Palomo; Marco Filice

doi:10.1016/j.biotechadv.2014.12.010

New emerging bio-catalysts design in biotransformations

Biotechnol Adv. 2015 Sep-Oct;33(5):605-13. doi: 10.1016/j.biotechadv.2014.12.010. Epub 2015 Jan 2.

Authors

Jose M Palomo¹, Marco Filice²

Affiliations

¹ Departamento of Biocatalisis, Instituto de Catalisis (CSIC), Marie Curie 2, Cantoblanco 28049, Madrid, Spain. Electronic address: josempalomo@icp.csic.es.
² Departamento of Biocatalisis, Instituto de Catalisis (CSIC), Marie Curie 2, Cantoblanco 28049, Madrid, Spain.

PMID: 25560932
DOI: 10.1016/j.biotechadv.2014.12.010

Abstract

The development of new and successful biotransformation processes of key interest in medicinal and pharmaceutical chemistry involves creating new biocatalysts with improved or even new activities and selectivities. This review emphasizes the new emerging developed strategies to achieve this goal, site-selective chemical modification of enzymes using tailor-made peptides, specific insertion of metals or organometallic complexes into proteins producing bio-catalysts with multiple activities and computational design for creating evolved artificial enzymes with non-natural synthetic catalytic activities.

Keywords: Artificial metalloenzymes; Bionanohybrids; Biotransformations; C–C bond reaction; Deacetylation; Regioselectivity; Semisynthetic enzymes.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Biocatalysis*
Biotechnology*
Biotransformation*
Enzymes* / chemistry
Enzymes* / metabolism
Protein Engineering
Stereoisomerism

Substances

Enzymes