Expression profile of Toll-like receptor mRNA in pigs co-infected with porcine reproductive and respiratory syndrome virus and porcine circovirus type 2

Pang-Yan Tu; Pei-Chun Tsai; Yi-Hsin Lin; Po-Cheng Liu; Hsiu-luan Chang; Tsun-Yung Kuo; Wen-Bin Chung

doi:10.1016/j.rvsc.2014.12.003

Expression profile of Toll-like receptor mRNA in pigs co-infected with porcine reproductive and respiratory syndrome virus and porcine circovirus type 2

Res Vet Sci. 2015 Feb:98:134-41. doi: 10.1016/j.rvsc.2014.12.003. Epub 2014 Dec 10.

Authors

Pang-Yan Tu¹, Pei-Chun Tsai¹, Yi-Hsin Lin¹, Po-Cheng Liu¹, Hsiu-luan Chang², Tsun-Yung Kuo³, Wen-Bin Chung⁴

Affiliations

¹ Department of Veterinary Medicine, National Pingtung University of Science and Technology, Neipu, Pingtung 912, Taiwan.
² Department of Animal Science, National Pingtung University of Science and Technology, Neipu, Pingtung 912, Taiwan. Electronic address: hlachang@mail.npust.edu.tw.
³ Department of Biotechnology and Animal Science, National Ilan University, Ilan 26041, Taiwan.
⁴ Department of Veterinary Medicine, National Pingtung University of Science and Technology, Neipu, Pingtung 912, Taiwan. Electronic address: wbchung@mail.npust.edu.tw.

PMID: 25555603
DOI: 10.1016/j.rvsc.2014.12.003

Abstract

Field and experimental studies have shown that co-infection of pigs with porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2) increases the severity of the disease. The present study investigates the mRNA expression profile of Toll-like receptors (TLRs) in pigs co-infected with PRRSV and PCV2. SPF pigs were infected with PRRSV, PCV2 or in a combination of both. The mRNA expression levels of TLRs and related cytokines in peripheral blood mononuclear cells (PBMCs) of pigs were determined by quantitative real-time RT-PCR. The mRNA expression profiles of TLRs by PBMCs from pigs co-infected with PRRSV and PCV2 displayed two distinct patterns: an increased expression profile for TLRs2, 4 and 8, and a decreased expression profile for TLRs3, 7 and 9. An up-regulated expression of IL-1β and IL-10 mRNA and a down-regulated expression of INF-α and TNF-α mRNA in PBMCs of co-infected pigs were also observed.

Keywords: Co-infection; Pigs; Porcine circovirus type 2; Porcine reproductive and respiratory syndrome virus; Toll-like receptors.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Circoviridae Infections / immunology
Circoviridae Infections / veterinary*
Circoviridae Infections / virology
Circovirus / immunology
Coinfection / immunology
Coinfection / veterinary*
Coinfection / virology
Cytokines / genetics
Cytokines / immunology
Gene Expression Profiling
Gene Expression Regulation / immunology*
Leukocytes, Mononuclear / immunology*
Leukocytes, Mononuclear / virology
Porcine Reproductive and Respiratory Syndrome / immunology*
Porcine Reproductive and Respiratory Syndrome / virology
Porcine respiratory and reproductive syndrome virus / immunology
RNA, Messenger / genetics
RNA, Messenger / metabolism
Random Allocation
Swine
Swine Diseases / immunology*
Swine Diseases / virology
Toll-Like Receptors* / genetics
Toll-Like Receptors* / immunology

Substances

Cytokines
RNA, Messenger
Toll-Like Receptors