Dendritic glycopolymers as dynamic and covalent coating in capillary electrophoresis: view on protein separation processes and detection of nanogram-scaled albumin in biological samples

Nikita Polikarpov; Vera Potolytsyna; Elena Bessonova; Sandra Tripp; Dietmar Appelhans; Brigitte Voit; Ludmilla Kartsova

doi:10.1016/j.chroma.2014.11.051

Dendritic glycopolymers as dynamic and covalent coating in capillary electrophoresis: view on protein separation processes and detection of nanogram-scaled albumin in biological samples

J Chromatogr A. 2015 Jan 23:1378:65-73. doi: 10.1016/j.chroma.2014.11.051. Epub 2014 Dec 12.

Authors

Nikita Polikarpov¹, Vera Potolytsyna², Elena Bessonova³, Sandra Tripp⁴, Dietmar Appelhans⁵, Brigitte Voit⁴, Ludmilla Kartsova⁶

Affiliations

¹ Leibniz Institute of Polymer Research Dresden, Hohe Straße 6, D-01069 Dresden, Germany(1); Organic Chemistry of Polymers, Technische Universität Dresden, D-01062 Dresden, Germany; Organic Chemistry Department, Saint Petersburg State University, Universitetsky Prospekt 26, Peterhof, 198504 Saint Petersburg, Russia.
² Organic Chemistry Department, Saint Petersburg State University, Universitetsky Prospekt 26, Peterhof, 198504 Saint Petersburg, Russia.
³ Organic Chemistry Department, Saint Petersburg State University, Universitetsky Prospekt 26, Peterhof, 198504 Saint Petersburg, Russia. Electronic address: bessonova.elena.a@gmail.com.
⁴ Leibniz Institute of Polymer Research Dresden, Hohe Straße 6, D-01069 Dresden, Germany(1); Organic Chemistry of Polymers, Technische Universität Dresden, D-01062 Dresden, Germany.
⁵ Leibniz Institute of Polymer Research Dresden, Hohe Straße 6, D-01069 Dresden, Germany(1).
⁶ Organic Chemistry Department, Saint Petersburg State University, Universitetsky Prospekt 26, Peterhof, 198504 Saint Petersburg, Russia. Electronic address: kartsova@gmail.com.

PMID: 25555410
DOI: 10.1016/j.chroma.2014.11.051

Abstract

Unique properties of dendritic polymers make them promising candidates for application as additives in various analytical methods. From this point, we investigated the potential use of maltose-modified hyperbranched poly(ethylene imine) (PEI-Mal) as a dynamically or covalently bound coating and a pseudostationary phase in capillary electrophoresis. The EOF mobilities were measured at different pH values (2.2, 8.5, and 10.2) using PEI-Mal in the background electrolyte (BGE) and desirable repeatability of the EOF with % RSD (n=50) ≤3.3 was obtained. The influence of pH, polymer concentration, and density of maltose shell on the separation properties of a model mixture of four proteins (albumin, lysozyme, myoglobin, insulin) were investigated. Applying PEI-Mal as a dynamic coating, improved separation of the protein mixture with a high repeatability was achieved. Applying PEI-Mal as a covalent coating for concentrating proteins in the large volume sample stacking (LVSS) combined with the field-enhanced sample injection (FESI), up to 1320-fold enhancement of sensitivity was achieved. The detection limit of 100-500ng/ml allowed successful analysis of albumin level both in blood and urine samples without additional preconcentration.

Keywords: Capillary electrophoresis; Covalent coating; Dynamic coating; Hyperbranched polymers; On-line concentration; Proteins.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Albumins / analysis*
Chemistry Techniques, Analytical / methods*
Electrolytes / chemistry
Electrophoresis, Capillary*
Limit of Detection
Polymers / chemistry
Proteins / chemistry

Substances

Albumins
Electrolytes
Polymers
Proteins