Highly sensitive and selective detection of miRNA: DNase I-assisted target recycling using DNA probes protected by polydopamine nanospheres

Yi Xie; Xiaoyan Lin; Yishun Huang; Rujun Pan; Zhi Zhu; Leiji Zhou; Chaoyong James Yang

doi:10.1039/c4cc08912j

Highly sensitive and selective detection of miRNA: DNase I-assisted target recycling using DNA probes protected by polydopamine nanospheres

Chem Commun (Camb). 2015 Feb 7;51(11):2156-8. doi: 10.1039/c4cc08912j. Epub 2015 Jan 2.

Authors

Yi Xie¹, Xiaoyan Lin, Yishun Huang, Rujun Pan, Zhi Zhu, Leiji Zhou, Chaoyong James Yang

Affiliation

¹ MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, Collaborative Innovation Center of Chemistry for Energy Materials, Key Laboratory of Chemical Biology of Fujian Province, State Key Laboratory of Physical Chemistry of Solid Surfaces, and Department of Chemistry, College of Chemistry & Chemical Engineering, Xiamen University, Xiamen 361005, P. R. China. cyyang@xmu.edu.cn ljzhou@xmu.edu.cn.

PMID: 25554948
DOI: 10.1039/c4cc08912j

Abstract

Based on the protective properties of polydopamine nanospheres for DNA probes against nuclease digestion, we have developed a DNase I-assisted target recycling signal amplification method for highly sensitive and selective detection of miRNA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biosensing Techniques / methods*
Cell Line, Tumor
DNA Probes / chemistry*
DNA Probes / genetics
Deoxyribonuclease I / metabolism*
Drug Carriers / chemistry
Humans
Indoles / chemistry*
Limit of Detection*
MicroRNAs / genetics
MicroRNAs / metabolism*
Models, Molecular
Nanospheres / chemistry*
Nucleic Acid Amplification Techniques
Nucleic Acid Conformation
Polymers / chemistry*

Substances

DNA Probes
Drug Carriers
Indoles
MicroRNAs
Polymers
polydopamine
Deoxyribonuclease I