Based on the reflected longitudinal wave, a new non-intrusive method for pressure measurement is proposed. The acoustoelastic theory and the thin-shell theory are introduced to develop the pressure measurement model in cylindrical pressure vessels. And a pressure measurement system is constructed to evaluate the effectiveness of the proposed method. The pressure measurement is implemented by measuring the travel-time change between two received ultrasonic sensors. The experimental results verify the feasibility and effectiveness of this new non-intrusive method. Compared with the non-intrusive pressure measurement method based on the critically refracted longitudinal wave (LCR wave), the proposed non-intrusive pressure measurement method has the advantages of higher sensitivity and higher signal-to-noise ratio.