Fermentative hydrogen and methane cogeneration from cassava residues: effect of pretreatment on structural characterization and fermentation performance

Jun Cheng; Richen Lin; Lingkan Ding; Wenlu Song; Yuyou Li; Junhu Zhou; Kefa Cen

doi:10.1016/j.biortech.2014.12.050

Fermentative hydrogen and methane cogeneration from cassava residues: effect of pretreatment on structural characterization and fermentation performance

Bioresour Technol. 2015 Mar:179:407-413. doi: 10.1016/j.biortech.2014.12.050. Epub 2014 Dec 19.

Authors

Jun Cheng¹, Richen Lin², Lingkan Ding², Wenlu Song³, Yuyou Li⁴, Junhu Zhou², Kefa Cen²

Affiliations

¹ State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China. Electronic address: juncheng@zju.edu.cn.
² State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China.
³ State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China; Department of Life Science and Engineering, Jining University, Jining 273155, China.
⁴ Department of Civil and Environmental Engineering, Tohoku University, Sendai 9808579, Japan.

PMID: 25553572
DOI: 10.1016/j.biortech.2014.12.050

Abstract

The physicochemical properties of cassava residues subjected to microwave (or steam)-heated acid pretreatment (MHAP or SHAP) were comparatively investigated to improve fermentative hydrogen and methane cogeneration. The hydrogen yield from cassava residues with MHAP and enzymolysis was higher (106.2 mL/g TVS) than that with SHAP and enzymolysis (102.1 mL/g TVS), whereas the subsequent methane yields showed opposite results (75.4 and 93.2 mL/g TVS). Total energy conversion efficiency increased to 24.7%. Scanning electron microscopy images revealed MHAP generated numerous regular micropores (∼6 μm) and SHAP generated irregular fragments (∼23 μm) in the destroyed lignocellulose matrix. Transmission electron microscopy images showed SHAP generated wider cracks (∼0.2 μm) in delaminated cell walls than MHAP (∼0.1 μm). X-ray diffraction patterns indicated MHAP caused a higher crystallinity index (33.00) than SHAP (25.88), due to the deconstruction of amorphous cellulose. Fourier transform infrared spectroscopy indicated MHAP caused a higher crystallinity coefficient (1.20) than SHAP (1.12).

Keywords: Cassava residue; Hydrogen; Methane; Microwave-heated acid; Steam-heated acid.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biotechnology / methods*
Carbohydrate Metabolism
Carbohydrates / analysis
Darkness
Fermentation*
Hydrogen / metabolism*
Manihot / chemistry*
Manihot / ultrastructure
Methane / biosynthesis*
Spectroscopy, Fourier Transform Infrared
X-Ray Diffraction

Substances

Carbohydrates
Hydrogen
Methane