A novel erythromycin resistance plasmid from Bacillus sp. strain HS24, isolated from the marine sponge Haliclona simulans

Teresa M Barbosa; Robert W Phelan; Dara Leong; John P Morrissey; Claire Adams; Alan D W Dobson; Fergal O'Gara

doi:10.1371/journal.pone.0115583

A novel erythromycin resistance plasmid from Bacillus sp. strain HS24, isolated from the marine sponge Haliclona simulans

PLoS One. 2014 Dec 30;9(12):e115583. doi: 10.1371/journal.pone.0115583. eCollection 2014.

Authors

Teresa M Barbosa¹, Robert W Phelan², Dara Leong³, John P Morrissey⁴, Claire Adams², Alan D W Dobson⁴, Fergal O'Gara⁵

Affiliations

¹ School of Pharmacy, University College Cork, Cork, Ireland.
² Department of Microbiology, University College Cork, Cork, Ireland; Biomerit Research Centre, University College Cork, Cork, Ireland.
³ Department of Microbiology, University College Cork, Cork, Ireland.
⁴ Department of Microbiology, University College Cork, Cork, Ireland; Marine Biotechnology Centre, Environmental Research Institute, University College Cork, Cork, Ireland.
⁵ Department of Microbiology, University College Cork, Cork, Ireland; Biomerit Research Centre, University College Cork, Cork, Ireland; Marine Biotechnology Centre, Environmental Research Institute, University College Cork, Cork, Ireland; Curtin University, School of Biomedical Sciences, Perth WA 6845, Australia.

Abstract

A better understanding of the origin and natural reservoirs of resistance determinants is fundamental to efficiently tackle antibiotic resistance. This paper reports the identification of a novel 5.8 kb erythromycin resistance plasmid, from Bacillus sp. HS24 isolated from the marine sponge Haliclona simulans. pBHS24B has a mosaic structure and carries the erythromycin resistance gene erm(T). This is the first report of an erythromycin resistance plasmid from a sponge associated bacteria and of the Erm(T) determinant in the genus Bacillus.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Aquatic Organisms / microbiology*
Bacillus / genetics*
Base Sequence
Drug Resistance, Bacterial / genetics*
Erythromycin*
Molecular Sequence Data
Plasmids / genetics*
Porifera / microbiology*

Substances

Erythromycin

Associated data

GENBANK/JF803858
GENBANK/KC991136

Grants and funding

This research was supported in parts by grants awarded to FOG by the Science Foundation of Ireland (SSPC2 12/RC/2275, 13-TIDA-B2625, 07/IN.1/B948, 12/TIDA/B2411, 12/TIDA/B2405,09/RFP/BMT 2350); the Department of Agriculture, Fisheries and Food (DAFF11/F/009 MabS, FIRM/RSF/CoFoRD; FIRM 08/RDC/629); the Environmental Protection Agency (EPA 2008-PhD/S-2), the Irish Research Council for Science, Engineering and Technology (PD/2011/2414; RS/2010/2413), the European Commission (FP7-PEOPLE-2013-ITN, 607786; OCEAN2012, 287589; FP7-KBBE-2012-6, CP-TP 311975; FP7-KBBE-2012-6, CP-TP-312184; Marie Curie 256596); and the Marine Institute (Beaufort award C2CRA 2007/082); Teagasc (Walsh Fellowship 2013) and the Health Research Board (HRA/2009/146). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.