[Establishment of real-time fluorescent quantitative polymerase chain reaction for rapid detection of M244V mutation in kinase domain of BCR-ABL fusion gene]

Qi Huang; Xin DU; Qiao-Xia Zhang; Jia-Cai Zhuo

doi:10.7534/j.issn.1009-2137.2014.06.042

[Establishment of real-time fluorescent quantitative polymerase chain reaction for rapid detection of M244V mutation in kinase domain of BCR-ABL fusion gene]

Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2014 Dec;22(6):1728-34. doi: 10.7534/j.issn.1009-2137.2014.06.042.

[Article in Chinese]

Authors

Qi Huang¹, Xin DU¹, Qiao-Xia Zhang², Jia-Cai Zhuo¹

Affiliations

¹ Department of Hematology, Guangzhou Medical University,The First Affiliated Hospital of Shenzhen University, The Second People's Hospital of Shenzhen, Shenzhen 518035, Guangdong Province, China.
² The First Affiliated Hospital of Shenzhen University, Shenzhen Blood Institute, Shenzhen 518035, Guangdong Province, China.

PMID: 25543505
DOI: 10.7534/j.issn.1009-2137.2014.06.042

Abstract

The present study was aimed to establish a high sensitive and specific method for detecting M244V mutation in kinase domain of BCR-ABL(fusion gene) by using real-time quantitative PCR technology. The specific primer of the mutational site was designed, and then the PCR reaction system and condition were optimized to establish the new real-time PCR method for detecting M244V mutation. The results showed that a method of detecting M244V mutation has been successfully established. The detection results indicated that this method possessed high sensitivity, specificity and accuracy. It is concluded that the method based on fluorescent quantitative polymerase chain reaction for detecting M244V mutation can be used to detect the M244V mutation in CML patients successfully.

MeSH terms

DNA Primers
Fusion Proteins, bcr-abl / genetics*
Humans
Mutation*
Real-Time Polymerase Chain Reaction / methods*

Substances

DNA Primers
Fusion Proteins, bcr-abl